Results 121 to 130 of about 633,065 (305)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Moving beyond animal models: enriched environments and human substance use disorders

Frontiers in Behavioral Neuroscience
BackgroundPreclinical studies have shown that exposure to a multisensory, stimulating environment (environmental enrichment, EE) can prevent the development of addictive behaviors and reduce the risk of relapse in animal models.
Lila Barillot, Lila Barillot, Claudia Chauvet, Emeline Chauchard, Marc Besnier, Marc Besnier, Ghina Harika-Germaneau, Ghina Harika-Germaneau, Xavier Noël, Nemat Jaafari, Nemat Jaafari, Marcello Solinas, Marcello Solinas, Armand Chatard, Armand Chatard   +14 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Comparative characteristics of quantitative and structural chromosome abnormalities at the time of diagnosis and in relapses of acute myeloid leukemias

Medičnì Perspektivi
Acute myeloid leukemias (AML) are a heterogeneous group of neoplasms of hematopoietic tissue, to determine the subtypes of which cytomorphological and cytochemical methods, immunophenotyping, cytogenetic and molecular genetic studies are carried out ...
S.V. Andreieva , K.V. Korets , I.M. Skorokhod , I.R. Hartovska, U.I. Melnyk   +4 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Catalpol Enhances Osteogenic Differentiation of Human Periodontal Stem Cells and Modulates Periodontal Tissue Remodeling in an Orthodontic Tooth Movement Rat Model

Drug Design, Development and Therapy
Jing Hu, Yang Song, Yuxing Zhang, Peng Yang, Siyu Chen, Zhaoyan Wu, Jun Zhang Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration ...
Hu J, Song Y, Zhang Y, Yang P, Chen S, Wu Z, Zhang J   +6 more
doaj  

Understanding Relapse in Bipolar Disorder at a Tertiary Mental Health Facility in Uganda [PDF]

Peter Kimbowa, Viola Nalwoga, Shaluwa Namwanje, Jovan Kisakye Ssekulima, Sharon Wawa Anguparu Department of Psychiatry, Lira University, Lira, Northern UgandaCorrespondence: Peter Kimbowa, Lira University, Faculty of Medicine, Department of Psychiatry, P.
Namwanje S, Nalwoga V, Kimbowa P, Anguparu SW, Ssekulima JK   +4 more
core  

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Relapse-free survival curves.

, 2016
The number of patients at risk at different time points are given below the graphs.
Martin Schumacher (50493), Richard F. Schlenk (2736112), Stefanie Hieke (2736115), Lars Bullinger (2736118), Axel Benner (30194), Harald Binder (39439)   +5 more
core   +1 more source