Results 91 to 100 of about 495,570 (281)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Variação diária e estacional do microclima urbano em ruas arborizadas de Curitiba-PR

open access: yesFloresta e Ambiente
O objetivo desta pesquisa foi analisar a tendência das variáveis meteorológicas em ruas de Curitiba-PR, com e sem arborização. Para tanto, foram selecionados trechos (arborizados e sem arborização) de ruas dos bairros Alto da Rua XV, Hugo Lange e ...
Angeline Martini   +2 more
doaj   +1 more source

Trend Analysis of Temperature and Humidity in Kwara State, Nigeria

open access: yesJournal of Environmental Geography, 2020
This paper examines the trend analysis of temperature and relative humidity in Kwara State. Climatic data on annual mean temperature (minimum and maximum) and relative humidity for 40 years (1978-2017) were collected from Nigerian Meteorological Agency ...
Adedapo Adeniyi
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Effect of relative humidity on the peeling behavior of a thin film on a rigid substrate

open access: yes, 2016
Inspired by gecko adhesion in humid environments, a modified Kendall's model is established in order to investigate the effect of relative humidity on the interfacial peeling behavior of a thin film adhering on a rigid substrate.
Chen, SH (reprint author), Beijing Inst Technol, Inst Adv Struct Technol, Beijing 100081, Peoples R China.   +4 more
core   +1 more source

An investigation into the effects of solvent content on the image quality and stability of ink jet digital prints under varied storage conditions [PDF]

open access: yes, 2010
Increasing numbers of galleries, museums and archives are including ink jet printed materials into their collections, and therefore displays. There is evidence that the instability of these prints is such that images can suffer deterioration in print ...
Sandy, Mark   +5 more
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

open access: yesFEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji   +2 more
wiley   +1 more source

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