Results 91 to 100 of about 224,841 (297)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Experimental Investigation on Spectral Linewidth and Relative Intensity Noise of High-Power Single-Frequency Polarization-Maintained Thulium-Doped Fiber Amplifier

open access: yesIEEE Photonics Journal, 2016
We investigate the spectral linewidth and relative intensity noise (RIN) of an all-fiber single-frequency polarization-maintained master oscillator and power amplifier at 1925 nm.
Haiwei Zhang   +6 more
doaj   +1 more source

Relative Intensity Noise Transfer Reduction in Raman-Assisted BOTDA Systems [PDF]

open access: yesIEEE Photonics Technology Letters, 2014
European ...
Angulo Vinuesa, Xabier   +5 more
openaire   +2 more sources

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Effect of Relative Intensity Noise in Optical Transmission Systems Employing Wavelength Conversion Based on XPM and MZI [PDF]

open access: yes, 2005
The overall intensity noise spectrum due to laser relative intensity noise (RIN) and interferometric conversion of laser phase noise after passing through wavelength conversion (WC) based on cross-phase modulation (XPM) and Mach-Zehnder interferometer ...
Chuah, Hean Teik   +2 more
core   +1 more source

Experimental Studies on the Noise Properties of the Harmonics From a Passively Mode-Locked Er-Doped Fiber Laser

open access: yesIEEE Photonics Journal, 2019
We experimentally investigate the noise properties of a homemade 586 MHz mode-locked laser (MLL). The variation of the timing jitter versus the harmonic order is measured, which is consistent with the theoretical analyses.
Jiazheng Song   +6 more
doaj   +1 more source

Effect of Optical Feedback on the Modulation Properties of AlGaInP Semiconductor Laser [PDF]

open access: yesمجلة التربية والعلم, 2008
Effect of optical feedback (O.F) on AlGaInP semiconductor laser properties and intensity modulation has been studied experimentally. Threshold current of semiconductor laser decreased 1mA when O.F equal to 160W , while there is no significant decreases ...
Musab Ahmed
doaj   +1 more source

Frequency Response of the Noise Conversion From Relative Intensity Noise to Phase Noise in the Photodetection of an Optical Pulse Train [PDF]

open access: yesIEEE Photonics Technology Letters, 2011
The frequency response of the noise conversion from the relative intensity noise (RIN) to the phase noise in the photodetection process of an optical pulse train is characterized with pump modulation method. It is found that the conversion ratio from the RIN to the phase noise is not sensitive to the noise power or the incident optical power, but ...
Wu, Kan   +4 more
openaire   +3 more sources

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

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