Results 121 to 130 of about 5,372,645 (294)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Nauka ta progres transportu, 2010 The class of mathematical modeling tasks of building constructions reliability, wherein the model of the «weakest link» can be used, is determined. The method of using this model is examined on the example of formation of reliability indices of steel ...
S. S. Nasonova
doaj
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Comparison of head-mounted perimeter (imo®) and Humphrey Field Analyzer
Clinical Ophthalmology, 2019 Tairo Kimura,1 Chota Matsumoto,2 Hiroki Nomoto2 1Department of Ophthalmology, Meiikai Ueno Eye Clinic, Tokyo, Japan; 2Department of Ophthalmology, Faculty of Medicine Osaka-Sayama City, Kindai University, Osaka, Japan Purpose: The head-mounted ...
Kimura T, Matsumoto C, Nomoto H
doaj
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
BuildingsFor the purpose of reliability quantitative assessment of the surrounding rock of the deeply embedded karst tunnel and the geological body around the cavern in the case of the cavern in the forepart of the tunnel face, on the basis of the upper bound ...
Bo Wu, Wentao Sun, Guowang Meng
doaj +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Temporal Network Reliability in Perturbed Scenarios: Application to a SCADA System
, 2012 The role of network reliability in the analysis of Critical Infrastructures (CI) is investigated showing that the traditional approach must be extended in two directions: to include the packet propagation time along the links for real time analysis, and ...
Bonaventura, Alessandro +15 more
core +1 more source