Results 101 to 110 of about 817,676 (313)

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome [PDF]

, 2007
The severe acute respiratory syndrome coronavirus (SARS-CoV) genome is predicted to encode 14 functional open reading frames, leading to the expression of up to 30 structural and non-structural protein products.
Brunn, Albrecht von, Teepe, Carola, Caroline C Friedel, Ralph Baric, Haas, Jürgen, von Brunn, Albrecht, Simpson Jeremy C., Jeremy C. Simpson, Simpson, Jeremy C., Ralf Zimmer, Roberts, Rhonda, Carola Teepe, Rainer Pepperkok, Baric Ralph, Haas Jürgen, Simpson, Jeremy, Zimmer, Ralf, Zimmer Ralf, Caroline C. Friedel, Friedel, Caroline C., Haas, Juergen, Teepe Carola, Albrecht von Brunn, Rhonda Roberts, Jürgen Haas, Friedel Caroline C., Pepperkok Rainer, Pepperkok, Rainer, Roberts Rhonda, Baric, Ralph, Jeremy C Simpson, von Brunn Albrecht   +31 more
core   +1 more source

Noroviruses Co-opt the Function of Host Proteins VAPA and VAPB for Replication via a Phenylalanine–Phenylalanine-Acidic-Tract-Motif Mimic in Nonstructural Viral Protein NS1/2

mBio, 2017
The Norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. Murine noroviruses provide the opportunity to study norovirus replication in cell culture and in small animals.
Broc T. McCune, Wei Tang, Jia Lu, James B. Eaglesham, Lucy Thorne, Anne E. Mayer, Emily Condiff, Timothy J. Nice, Ian Goodfellow, Andrzej M. Krezel, Herbert W. Virgin   +10 more
doaj   +1 more source

Inhibition of IKKα by BAY61-3606 Reveals IKKα-Dependent Histone H3 Phosphorylation in Human Cytomegalovirus Infected Cells. [PDF]

PLoS ONE, 2016
Protein kinase inhibitors can be used as tools to identify proteins and pathways required for virus replication. Using virus replication assays and western blotting we found that the widely used protein kinase inhibitor BAY61-3606 inhibits replication of
Catherine M K Ho, I'ah Z Donovan-Banfield, Li Tan, Tinghu Zhang, Nathanael S Gray, Blair L Strang   +5 more
doaj   +1 more source

Protein-Primed Replication of Bacteriophage Φ29 DNA [PDF]

, 2011
The requirement of DNA polymerases for a 3'-hydroxyl (3'-OH) group to prime DNA synthesis raised the question about how the ends of linear chromosomes could be replicated. Among the strategies that have evolved to handle the end replication problem, a group of linear phages and eukaryotic and archaeal viruses, among others, make use of a protein ...
M, Salas, M, de Vega
openaire   +2 more sources

Repair-specific Functions of Replication Protein A [PDF]

Journal of Biological Chemistry, 2012
Replication protein A (RPA), the major eukaryotic single-strand DNA (ssDNA)-binding protein, is essential for replication, repair, recombination, and checkpoint activation. Defects in RPA-associated cellular activities lead to genomic instability, a major factor in the pathogenesis of cancer and other diseases.
Cathy S, Hass, Koonyee, Lam, Marc S, Wold   +2 more
openaire   +2 more sources

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Role of the RPA-Sgs1 interaction in stabilizing stalled replication forks [PDF]

, 2011
S phase is the period of the cell cycle when all genomic DNA is copied precisely twice. During this complex process, replication forks frequently encounter obstacles such as tightly bound protein-barriers or are challenged by genotoxic insults creating ...
Hegnauer, Anna Maria
core   +1 more source

Heat shock protein A1L restricts influenza A virus by ubiquitination of NA

Journal of Virology
Neuraminidase (NA) protein of influenza A virus (IAV), a tetrameric envelope glycoprotein critical for viral release and spread, has well-characterized enzymatic functions but poorly resolved host-pathogen interactions.
Yan Yan, Jianan Xu, Zhen Chen, Yuting Xu, Linlong Qin, Lingyan Zhao, Hongli Zhang, Xiaoxiao Feng, Chaoliang Yao, Yu Huang, Jiyong Zhou, Tingjuan Deng   +11 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source