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BioTechnologia, 2015 Some of the next articles are maybe not open access.
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American Journal of Roentgenology, 2013
The purposes of this article are to summarize the basic concept and the strategies of reporter imaging; introduce reporter genes frequently used in optical imaging, nuclear medicine, and MRI for in vivo application; and show typical examples of reporter gene imaging.In molecular biology, many reporter genes have been developed for monitoring cellular ...
Hyewon Youn, June-Key Chung
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The purposes of this article are to summarize the basic concept and the strategies of reporter imaging; introduce reporter genes frequently used in optical imaging, nuclear medicine, and MRI for in vivo application; and show typical examples of reporter gene imaging.In molecular biology, many reporter genes have been developed for monitoring cellular ...
Hyewon Youn, June-Key Chung
semanticscholar +3 more sources
Reporter gene expression for monitoring gene transfer
Current Opinion in Biotechnology, 1997The use of reporters such as green fluorescent protein (GFP) and firefly luciferase permit highly sensitive and nondestructive monitoring of gene transfer and expression. Modifications in GFP which increase intensity and thermostability, as well as alter its spectral qualities, have facilitated the use of GFP in a variety of gene transfer methods ...
S, Welsh, S A, Kay
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Monitoring gene therapy with reporter gene imaging
Seminars in Nuclear Medicine, 2001Rapid advances in imaging technologies and gene transfer strategies offer a great opportunity to optimize clinical trials of human gene therapy. Reporter genes are emerging as very powerful tools to monitor the delivery, magnitude, and time variation of therapeutic gene transfer in vivo.
P, Ray +7 more
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2010
In recent years, several reporter genes have been developed that can serve as a beacon for non-invasive magnetic resonance imaging (MRI). Here, we provide a brief summary of recent advances in MRI reporter gene technology, as well as detailed "hands-on" protocols for cloning, expression, and imaging of reporter genes based on chemical exchange ...
Guanshu, Liu +2 more
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In recent years, several reporter genes have been developed that can serve as a beacon for non-invasive magnetic resonance imaging (MRI). Here, we provide a brief summary of recent advances in MRI reporter gene technology, as well as detailed "hands-on" protocols for cloning, expression, and imaging of reporter genes based on chemical exchange ...
Guanshu, Liu +2 more
openaire +2 more sources
2006
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern +2 more
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Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern +2 more
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Reporter Gene Vectors and Assays
Molecular Biotechnology, 1999Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems.
E, Schenborn, D, Groskreutz
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Gene manipulation success reported
Nursing Standard, 1990For the first time foreign genes have been inserted into the human body.
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Reporter genes in transgenic mice
Transgenic Research, 1994Although in vivo models utilizing endogenous reporter genes have been exploited for many years, the use of reporter transgenes to dissect biological issues in transgenic animals has been a relatively recent development. These transgenes are often, but not always, of prokaryotic origin and encode products not normally associated with eukaryotic cells ...
C, Cui +4 more
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Radionuclide reporter gene imaging for cardiac gene therapy
European Journal of Nuclear Medicine and Molecular Imaging, 2007In the field of cardiac gene therapy, angiogenic gene therapy has been most extensively investigated. The first clinical trial of cardiac angiogenic gene therapy was reported in 1998, and at the peak, more than 20 clinical trial protocols were under evaluation.
Masayuki, Inubushi, Nagara, Tamaki
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