Results 101 to 110 of about 1,854,175 (305)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins [PDF]

, 2006
BACKGROUND: CtBP1 and CtBP2 are transcriptional co-repressors that modulate the activity of a large number of transcriptional repressors via the recruitment of chromatin modifiers.
Bergman, Lee M., Jeremy P Blaydes, Mirnezami Alexander H, Alexander H Mirnezami, Bergman Lee M, Samal C Gunatilake, Mirnezami, Alexander H., Darley Matthew, Morris Laila, Lee M Bergman, Laila Morris, Darley, Matthew, Gunatilake, Samal C., Blaydes, Jeremy P., Morris, Laila, Matthew Darley, Gunatilake Samal C, Blaydes Jeremy P   +17 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Analysis of the interaction of TIP60β and PIN1 with the ets family transcription factor ETV6 [PDF]

, 2005
The ETV6 gene is involved in many chromosomal translocations forming different fusion genes in both myeloid and lymphoid leukemias as well as in some solid tumors.
Zhang, Chang-Dong
core   +1 more source

The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation

BMC Plant Biology, 2008
Background In Arabidopsis, INDOLE-3-BUTYRIC ACID RESPONSE5 (IBR5), a putative dual-specificity protein phosphatase, is a positive regulator of auxin response.
Bartel Bonnie, Monroe-Augustus Melanie, Strader Lucia C   +2 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Targeting C-terminal binding proteins (CtBPs) using genetic selection

, 2011
There are many protein-protein interactions that are vital for cellular processes such as signal transduction, structural organisation and apoptosis. In this study we decipher the role of the protein-protein interaction of C terminal Binding Proteins ...
Nijjar, Sharandip K.
core   +1 more source

Data on the target search by a single protein on DNA measured with ultrafast force-clamp spectroscopy

Data in Brief, 2019
The mechanism by which proteins are able to find small cognate sequences in the range from few to few tens of base pairs amongst the millions of non-specific chromosomal DNA has been puzzling researchers for decades.
Carina Monico, Alessia Tempestini, Lucia Gardini, Francesco Saverio Pavone, Marco Capitanio   +4 more
doaj   +1 more source

Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus. Co-purification of RNase A with Sso7c.

, 1998
The thermostable histone-like protein Sso7c (Sso for Sulfolobus solfataricus) from the archaeon Sulfolobus solfataricus was purified from the supernatant of acid-soluble cell lysates. Reverse phase HPLC of an apparently homogeneous Sso7c protein fraction
Knapp, Stefan, Stefan Knapp, Oppermann, UC, Bonetto, V, Hans Jörnvall, Jörnvall, H, Ladenstein, R, Rudolf Ladenstein, Udo C.T. Oppermann, Valentina Bonetto, Knapp, S, Ladenstein, R,, Jörnvall, H,, Oppermann, UCT   +13 more
core   +1 more source

Interaction of NCOR/SMRT Repressor Complexes with Papillomavirus E8^E2C Proteins Inhibits Viral Replication.

PLoS Pathogens, 2016
Infections with high-risk human papillomaviruses (HR-HPV) such as HPV16 and 31 can lead to ano-genital and oropharyngeal cancers and HPV types from the beta genus have been implicated in the development of non-melanoma skin cancer.
Marcel Dreer, Jasmin Fertey, Saskia van de Poel, Elke Straub, Johannes Madlung, Boris Macek, Thomas Iftner, Frank Stubenrauch   +7 more
doaj   +1 more source