Results 161 to 170 of about 166,851,649 (309)
Grand Rounds: Research Reproducibility - Brian Sauer PhD, MS (02-06-2018)
, 2018 "Spencer S. Eccles Health Sciences Library Grand Rounds: Research Reproducibility (GRRR). "Workflow Management and Research Reproducibility" presented by Brian C. Sauer, PhD, MS.
Brian Sauer, PhD, MS
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Grand Rounds: Research Reproducibility - Alan H. Morris, MD (03-06-2018)
, 2018 Spencer S. Eccles Health Sciences Library Grand Rounds: Research Reproducibility (GRRR). "A new Conceptual Model for Coordinating Different Strategies to Enhance Replicability." GRRR is a weekly endeavor to raise awareness about reproducibility issues ...
Alan H. Morris, MD
core
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Grand Rounds: Research Reproducibility - Julio C. Facelli, PhD (01-30-2018)
, 2018 Spencer S. Eccles Health Sciences Library Grand Rounds: Research Reproducibility (GRRR). "Uncertainty Quantification for Biomedical Decision Making: A Critical Informatics Contribution to Precision Medicine"" presented by Julio C.
Julio C. Facelli, PhD
core
Pesquisa Brasileira em Odontopediatria e Clínica IntegradaObjective: To evaluate the validity and reliability of Planas’ Functional Masticatory Angle (PFMA) method with a modified device compared to the clinical method as the gold standard for determining masticatory preference side in children.
María Claudia Reyes Márquez Márquez +6 more
doaj
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source