Results 111 to 120 of about 3,776,108 (289)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mutation, selection, and ancestry in branching models: a variational approach

, 2006
We consider the evolution of populations under the joint action of mutation and differential reproduction, or selection. The population is modelled as a finite-type Markov branching process in continuous time, and the associated genealogical tree is ...
A. Dembo, A. Edwards, C. Kamp, D. Mitrinovic, E. Akin, E. Baake, E. Baake, Ellen Baake, F. Crow J., F. den Hollander, H. Kesten, H.-O. Georgii, Hans-Otto Georgii, J. Hein, J. Hermisson, J. Hofbauer, J.G. Kemeny, K.B. Athreya, K.S. Karlin, M. Eigen, M. Eigen, P. Jagers, P. Jagers, P. Jagers, R. Bürger, R. Lyons, R.T. Rockafellar, T. Garske, T. Kurtz, T. Lindvall, U. Gerland, W. Ewens, W. Ewens, W. Stannat   +33 more
core   +3 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Elective single morula transfer achieved better clinical outcomes than two cleavage embryos transfer in fresh IVF-ET cycles

BMC Pregnancy and Childbirth
Background Assisted reproductive technology (ART) effectively treats infertility yet often results in multiple pregnancies. Optimizing clinical pregnancy rates while minimizing multiples remains challenging.
Qing Feng, Juan Liu, Zhiwei Zhou, Jinbao Ma, Yan Song, Zhaoxia Luo, Xiaohui Ye, Lang Shen   +7 more
doaj   +1 more source

Interrelationships between gut microbiome and serum medium mass molecules in menopausal women

Сибирский научный медицинский журнал
During menopause, the composition of the gut microbiota undergoes changes. At the same time, age-related deficiency of sex hormones occurs, which is considered the basis for the development of metabolic disorders, including oxidative and carbonyl stress,
N. E. Garashchenko, N. V. Semenova, N. L. Belkova, O. A. Nikitina, E. A. Novikova, N. E. Smurova, E. S. Klimenko, S. I. Kolesnikov, A. S. Lesnaya, L. I. Kolesnikova   +9 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

8-Hydroxy-2’-deoxyguanosine level and antioxidant status in the dynamics of physiological pregnancy

Acta Biomedica Scientifica
Background. Due to the adaptive processes occurring in a woman’s body during pregnancy, the antioxidant status may be the subject for active changes. An imbalance between antioxidants and prooxidants may lead to the development of oxidative stress.
O. A. Nikitina, N. V. Semenova, E. A. Novikova, A. N. Karacheva, A. S. Lesnaya, S. I. Kolesnikov, A. Yu. Marianian, N. V. Protopopova, L. I. Kolesnikova   +8 more
doaj   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Role of RGS12 in placental mitochondrial dysfunction and adverse pregnancy outcomes

Cellular and Molecular Life Sciences
Mitochondrial function and its regulation within the placenta are critical for maintaining a healthy pregnancy. This study investigated the role of G-protein signaling 12 (RGS12) in placental mitochondrial function and pregnancy outcomes. RGS12 was found
Xianling Cao, Xuanyou Zhou, Naixin Xu, Weihui Shi, Hefeng Huang, Zhengao Sun, Songchang Chen, Chen-Ming Xu   +7 more
doaj   +1 more source

HUBUNGAN ANTARA PENGETAHUAN KESEHATAN REPRODUKSI DAN KETAATAN BERAGAMA DENGAN SIKAP TERHADAP HUBUNGAN SEKS PRANIKAH (STUDI PADA SISWA KELAS II SMAN 1 SEMARANG) CORRELATION BETWEEN KNOWLEDGE OF REPRODUCTION HEALTH, RELIGIOUSNESS OBEDIENCE AND ATTITUDE TO PREMARITAL SEXUAL INTERCOURSE (STUDY ON THE SECOND GRADE STUDENTS OF SMAN 1 SEMARANG) [PDF]

, 2004
Kesehatan reproduksi remaja saat ini sudah menjadi isu global. Jumlah remaja yang besar dan secara seksual tergolong aktif, namun mempunyai pengetahuan kesehatan reproduksi yang relatif rendah sehingga kurang mendukung terciptanya remaja berkualitas ...
MUBAROK, MUH. AMIN
core