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Standard Operating Procedure to Optimize Resazurin-Based Viability Assays
BiosensorsThe resazurin assay, also known as the Alamar Blue assay, stands as a cornerstone technique in cell biology, microbiology, and drug development. It assesses the viability of cells through the conversion of resazurin into highly fluorescent resorufin. The
Laura Revel +2 more
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Revisiting the Resazurin-Based Sensing of Cellular Viability: Widening the Application Horizon
Biosensors, 2022 Since 1991, the NAD(P)H-aided conversion of resazurin to fluorescent resorufin has been widely used to measure viability based on the metabolic activity in mammalian cell culture and primary cells.
Darja Lavogina +2 more
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Molecules, 2023 Cell viability and metabolic activity are ubiquitous parameters used in biochemistry, molecular biology, and biotechnological studies. Virtually all toxicology and pharmacological projects include at some point the evaluation of cell viability and/or ...
Miguel A Castanho
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Bioengineering, 2023 The resazurin reduction test is one of the basic tests for bacterial culture viability and drug resistance endorsed by the World Health Organisation. At the same time, conventional spectrophotometric and spectrofluorimetric methods demand rather bulky ...
Alexander V. Sychev +3 more
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Employment of a Resazurin Viability-Based Assay for Minimum Inhibitory and Bactericidal Concentration Determination [PDF]
PharmaceuticalsBackground/Objectives: The increasing prevalence of antimicrobial-resistant bacteria highlights the need for improved methodologies to evaluate antimicrobial activity beyond conventional minimum inhibitory concentration testing.
Lorena G. Calvo +4 more
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Role of dipA and pilD in Francisella tularensis Susceptibility to Resazurin
Antibiotics, 2021 The phenoxazine dye resazurin exhibits bactericidal activity against the Gram-negative pathogens Francisella tularensis and Neisseria gonorrhoeae. One resazurin derivative, resorufin pentyl ether, significantly reduces vaginal colonization by Neisseria ...
Kendall Souder +11 more
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Quantifying metabolic activity of Ascaris suum L3 using resazurin reduction
Parasites & Vectors, 2023 Background Helminth infections are an important public health problem in humans and have an even greater impact on domestic animal and livestock welfare.
Arkadi Kundik +6 more
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Use of Resazurin To Rapidly Enumerate Bdellovibrio and Like Organisms and Evaluate Their Activities
Microbiology Spectrum, 2022 A method to rapidly quantify predatory bacterial cell populations using resazurin reduction to resorufin and its resulting fluorescence kinetics (dF/dt) are described.
Hyochan Jang +3 more
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Antibiotics, 2021 Bacterial inactivation using bacteriophages (or phages) has emerged as an effective solution for bacterial infections, but the screening methods used to evaluate the effectiveness of the phages to inactivate bacteria are not fast, reliable or precise ...
Pedro Costa +4 more
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Methods, 2007 The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts ...
Satyajit D Sarker, Lutfun Nahar
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