Results 51 to 60 of about 24,024 (231)

Evaluation of resazurin phenoxazine dye as a highly sensitive cell viability potency assay for natural killer cell‐derived extracellular vesicle‐based cancer biotherapeutics

Journal of Extracellular Biology
Natural killer cell‐derived extracellular vesicles (NK‐EVs) are candidate biotherapeutics against various cancers. However, standardised potency assays are necessary for a reliable assessment of NK‐EVs' cytotoxicity.
Frederic St-Denis-Bissonnette, Shirley Qiu, Sarah E. Cummings, Melanie Kirkby, Y. Haile, Sarah J. Wassmer, G. Muradia, Jelica Mehic, Andrew Stalker, A. Shrestha, Michele Ardolino, Seung-Hwan Lee, Dylan Burger, Lisheng Wang, Jessie R. Lavoie   +14 more
semanticscholar   +1 more source

Caveats to the use of MTT, neutral red, Hoechst and Resazurin to measure silver nanoparticle cytotoxicity.

Chemico-Biological Interactions, 2020
The extensive use of silver nanoparticles (AgNPs) in manufactured products will inevitably increase environmental exposure, highlighting the importance of accurate toxicity assessments.
D. F. Mello, R. Trevisan, N. Rivera, Nicholas K. Geitner, R. Di Giulio, M. Wiesner, Heileen Hsu-Kim, Joel N. Meyer   +7 more
semanticscholar   +1 more source

Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections [PDF]

Antimicrobial Agents and Chemotherapy, 2013
ABSTRACT The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen.
Osaka, Ichie, Hefty, P. Scott
openaire   +3 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria

BMC Microbiology, 2020
Colistin resistance is considered a serious problem due to a lack of alternative antibiotics. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is a resazurin reduction-based technique that relies on the visual detection of bacterial growth in ...
Huaiyu Jia, Renchi Fang, Jie Lin, Xuebin Tian, Ya-jie Zhao, Lijiang Chen, Jianming Cao, Tieli Zhou   +7 more
semanticscholar   +1 more source

Substrate Stress Relaxation Regulates Cell‐Mediated Assembly of Extracellular Matrix

Advanced Functional Materials, EarlyView.
Silicone‐based viscoelastic substrates with tunable stress relaxation reveal how matrix mechanics regulates cellular mechanosensing and cell‐mediated matrix remodelling in the stiff regime. High stress relaxation promotes assembly of fibronectin fibril‐like structures, increased nuclear localization of YAP and formation of β1 integrin‐enriched ...
Jonah L. Voigt, Jens Timmer, Elisabetta Ada Cavalcanti‐Adam   +2 more
wiley   +1 more source

Multiscale Structuring of Hydroxyapatite via Two‐Photon Lithography of Nanocomposites

Advanced Functional Materials, EarlyView.
Hydroxyapatite scaffolds are of great interest in bone tissue engineering applications, ranging from 3D cell culture to regenerative medicine. Using two‐photon lithography of a transparent nanocomposite, hydroxyapatite microstructures with features ranging from submicron to centimeter‐scale are fabricated. This allows to mimic the natural bone geometry,
Leonhard Hambitzer, Jan Mathis Hornbostel, Richard Prediger, Sebastian Kluck, Michael Daub, Cornelia Lee‐Thedieck, Frederik Kotz‐Helmer   +6 more
wiley   +1 more source

Comparison of Four Methods for the in vitro Susceptibility Testing of Dermatophytes

Frontiers in Microbiology, 2020
ObjectivesInfections caused by dermatophytes affect a high percentage of the population. Antifungal susceptibility testing (AST) can offer useful information about the susceptibility profiles of the pathogens as well as the concomitant documentation of ...
Anthi-Marina Markantonatou, Konstantinos Samaras, Evaggelia Zachrou, Timoleon-Achilleas Vyzantiadis   +3 more
doaj   +1 more source

Shaping Ti3C2 MXene Nanospheres for Precision Near‐Infrared Photothermal Therapy

Advanced Functional Materials, EarlyView.
In this study, we report producing spherical MXenes via fs laser fragmentation of Ti3C2 flakes in liquid medium. The nanoparticles demonstrated pronounced light absorption and high photothermal conversion efficiencies of 68% and 63% under heating with NIR‐I and NIR‐II lasers, respectively.
Julia S. Babkova, Ivan V. Zelepukin, Konstantin G. Shevchenko, Gleb V. Tikhonowski, Lyubov V. Gorelik, Anton A. Popov, Anna S. Sogomonyan, Alina Yu. Kapitannikova, Ivan S. Kazantsev, Dmitry V. Dyubo, Daria A. Panova, Daniil I. Tselikov, Anton A. Minnekhanov, Aziz B. Mirkasymov, Georgy A. Ermolaev, Alexander V. Syuy, Gleb I. Tselikov, Aleksey V. Arsenin, Andrei V. Kabashin, Kostya S. Novoselov, Sergey M. Deyev, Valentyn S. Volkov   +21 more
wiley   +1 more source

Dose–Response Curve in REMA Test: Determination from Smartphone-Based Pictures

Analytica
We report a workflow and a software description for digital image colorimetry aimed at obtaining a quantitative dose–response curve and the minimal inhibitory concentration in the Resazurin Microtiter Assay (REMA) test of the activity of ...
Eugene B. Postnikov, Alexander V. Sychev, Anastasia I. Lavrova   +2 more
doaj   +1 more source