Results 121 to 130 of about 2,222,500 (385)

Reducing Transfer of Salmonella and Aerobic Mesophilic Bacteria on Melon Rinds Surfaces to Fresh Juice by Washing With Chlorine: Effect of Waiting Period Before Refrigeration of Prepared Juice

Frontiers in Sustainable Food Systems, 2018
Cantaloupes, honeydew melons and watermelons inoculated with Salmonella cocktail at 4.5, 3.8, and 3.2 log10 CFU/cm2, respectively, were sanitized with 200 ppm chlorine before rinds removal, cutting, and juice preparation.
Dike O. Ukuku, Sudarsan Mukhopadhyay, Modesto Olanya   +2 more
doaj   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Dynamics underlie the drug recognition mechanism by the efflux transporter EmrE

Nature Communications
The multidrug efflux transporter EmrE from Escherichia coli requires anionic residues in the substrate binding pocket for coupling drug transport with the proton motive force. Here, we show how protonation of a single membrane embedded glutamate residue (
Jianping Li, Ampon Sae Her, Alida Besch, Belen Ramirez-Cordero, Maureen Crames, James R. Banigan, Casey Mueller, William M. Marsiglia, Yingkai Zhang, Nathaniel J. Traaseth   +9 more
doaj   +1 more source

Redox‐dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells

FEBS Letters, EarlyView.
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker, Nimit Mistry, Nicoletta Plotegher, Elizabeth R. Westbrook, Michael D. E. Sewell, John Carroll, Gyorgy Szabadkai, Angus J. Bain, Michael R. Duchen   +8 more
wiley   +1 more source

Rational design of a (S)-selective-transaminase for asymmetric synthesis of (1S)-1-(1,1′-biphenyl-2-yl)ethanamine [PDF]

, 2016
Amine transaminases offer an environmentally sustainable synthesis route for the production of pure chiral amines. However, their catalytic efficiency toward bulky ketone substrates is greatly limited by steric hindrance and therefore presents a great ...
Allen, Christopher C.R., Brown, Rodney T., Carvalho, Alexandra T.P., Caswell, Jill M., Dheeman, Dharmendra S., Dourado, Daniel F.A.R., Huang, Meilan, Kulakov, Leonid, Miskelly, Iain, Moody, Thomas S., Pohle, Stefan, Quinn, Derek J., Skvortsov, Timofey   +12 more
core   +3 more sources

Radical chemistry for selective modification of native residues in proteins

, 2020
The chemical modification of proteins has provided a toolbox enabling many experiments in chemical biology. However, new strategies are required to be able to specifically target (hetero)aromatic residues. Fluorine atoms installed in proteins can serve as useful tags, but methods for efficient and selective chemical fluorination are limited.
openaire   +2 more sources

Essential Trace Elements in Three Species of Dolphins Stranded in the Croatian Part of the Adriatic Sea from 1995 to 2013

Animals
Trace elements are widely distributed in the environment and are considered essential when their deficiency leads to impaired biological function. This study aimed to quantify concentrations of two essential trace elements—copper (Cu) and zinc (Zn)—in ...
Maja Đokić, Nina Bilandžić, Marija Sedak, Tomislav Bolanča, Tomislav Gomerčić, Martina Đuras, Miroslav Benić   +6 more
doaj   +1 more source

Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice

FEBS Letters, EarlyView.
In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász, Albert Bálint Papp, Lukács Sándor Lesinszki, Attila Mócsai, Tamás Varga, László Nagy, Péter Gogolák   +6 more
wiley   +1 more source

Thermal Decomposition and Combustion of γ-irradiated Polyamide 6 Containing Phosphorus Oxynitride or Phospham [PDF]

, 1999
Polyamide 6 (PA-6) containing the fire retardants phosphorus oxynitride ((PON)m) or phospham ((PN2H)n) was exposed to 60Co-γ-rays (absorbed dose: 1.0–4.0 MGy).
Balabanovich, A. I., Levchik, Galina F, Levchik, Sergei V, Schnabel, W., Wilkie, Charles   +4 more
core   +1 more source

Faithful Estimation of Dynamics Parameters from CPMG Relaxation Dispersion Measurements [PDF]

, 2006
This work examines the robustness of fitting of parameters describing conformational exchange (kex, pa/b, and Δω) processes from CPMG relaxation dispersion data.
Grey, Michael J., Kempf, James G., Kovriguine, Evgueni, Loria, J. Patrick   +3 more
core   +2 more sources