Results 41 to 50 of about 453,408 (276)
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Climate change and disaster impact reduction [PDF]
, 2008 Based on papers presented at the 'UK - South Asia Young Scientists and Practitioners Seminar on Climate Change and Disaster Impact Reduction' held at Kathmandu, Nepal on 5-6 June ...
Aryal, Komal, Gadema, Zaina
core
, 2014 We present a large-scale study of commonality in liquidity and resilience across assets in an ultra high-frequency (millisecond-timestamped) Limit Order Book (LOB) dataset from a pan-European electronic equity trading facility.
Kosmidis, Ioannis +2 more
core +1 more source
Ambient but not local lactate underlies neuronal tolerance to prolonged glucose deprivation [PDF]
, 2018 Neurons require a nearly constant supply of ATP. Glucose is the predominant source of brain ATP, but the direct effects of prolonged glucose deprivation on neuronal viability and function remain unclear.
Mennerick, Steven +3 more
core +3 more sources
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Living Shorelines & Resilience in Southern California: A Summary of a Series of Workshops held as part of The Resilient Coastlines Project of Greater San Diego [PDF]
, 2018 In 2016, a series of workshops were held to discuss living shorelines in SouthernCalifornia. The workshops specifically focused on the unique benefits, challenges andopportunities for implementing living shorelines in the region, and the nexus ...
Engeman, Laura
core
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Collective intelligence for OER sustainability [PDF]
, 2010 To thrive, the Open Educational Resource (OER) movement, or a given initiative, must make sense of a complex, changing environment. Since “sustainability” is a desirable systemic capacity that our community should display, we consider a number of ...
Buckingham Shum, Simon, De Liddo, Anna
core
Small cities face greater impact from automation
, 2017 The city has proven to be the most successful form of human agglomeration and provides wide employment opportunities for its dwellers. As advances in robotics and artificial intelligence revive concerns about the impact of automation on jobs, a question ...
Cebrian, Manuel +4 more
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source