Simple Universal Whole-Organ Resin-Embedding Protocol for Display of Anatomical Structures [PDF]
Biomedicines, 2023 Whole-organ plastic resin casting is a very useful method for preserving rare pathological specimens for forensic/anatomical studies and for teaching/research purposes.
Daniel Pirici +2 more
exaly +4 more sources
Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest [PDF]
Frontiers in Zoology, 2023 Background For decoding the mechanism of how cells and organs function information on their ultrastructure is essential. High-resolution 3D imaging has revolutionized morphology.
Barbora Konopová, Jiří Týč
doaj +2 more sources
Low-temperature resin embedding of the whole brain for various precise structures dissection [PDF]
iScience, 2023 Summary: Resin embedding combined with ultra-thin sectioning has been widely used in microscopic and electron imaging to acquire precise structural information of biological tissues.
Jiaojiao Tian +5 more
doaj +2 more sources
Scalable Resin Embedding Method for Large-Volume Brain Tissues with High Fluorescence Preservation Capacity [PDF]
iScience, 2020 Summary: Resin embedding is widely used to dissect the fine structure of bio-tissue with electron and optical microscopy. However, it is difficult to embed large-volume tissues with resin.
Ting Luo +8 more
doaj +2 more sources
Quick and easy sample preparation without resin embedding for the bone quality assessment of fresh calcified bone using fourier transform infrared imaging. [PDF]
PLoS ONE, 2018 Fourier transform infrared (FTIR) imaging is a powerful tool for the assessment of bone quality; however, it requires the preparation of thin bone sections.
Hiromi Kimura-Suda +6 more
doaj +2 more sources
Critical point drying of brain tissue for X-ray phase-contrast imaging [PDF]
Journal of Synchrotron RadiationX-ray phase-contrast tomography can efficiently image brain tissue at subcellular resolution. However, current sample preparation methods are not optimized to exploit the full potential of X-ray contrast mechanisms.
Safe Khan +15 more
doaj +3 more sources
High-Temperature-Resistant Composite Lost Circulation Materials for Oil-Based Drilling Fluids: Preparation, Performance, and Synergistic Mechanism [PDF]
MoleculesLost circulation in oil-based drilling fluids (OBDFs) under high-temperature conditions remains a significant challenge in deep and ultra-deep drilling.
Yue Gao +5 more
doaj +2 more sources
Immunoelectron microscopy: a comprehensive guide from sample preparation to high-resolution imaging [PDF]
Discover NanoImmunoelectron Microscopy (IEM) is a technique that combines specific immunolabeling with high-resolution electron microscopic imaging to achieve precise spatial localization of biomolecules at the subcellular scale (
Jinsai Wu +5 more
doaj +2 more sources
One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM
Frontiers in Cell and Developmental Biology, 2022 Sample preparation is the novel bottleneck for high throughput correlative light and electron microscopy (CLEM). Protocols suitable for both imaging methods must therefore balance the requirements of each technique.
Xavier Heiligenstein, Miriam S. Lucas
doaj +1 more source
Improved Fluorescent Proteins for Dual-Colour Post-Embedding CLEM
Cells, 2022 Post-embedding correlative light and electron microscopy (CLEM) has the advantage of high-precision registration and enables light and electron microscopy imaging of the same slice.
Dingming Peng +6 more
doaj +1 more source