Results 11 to 20 of about 29,160 (257)

Transverse Sectioning of Arabidopsis thaliana Leaves Using Resin Embedding [PDF]

open access: yesBio-Protocol, 2015
The leaf is the major functional part of the shoot performing the bulk of photosynthetic activity. Its development is relatively plastic allowing the plant to adapt to environmental changes by modifying leaf size and anatomy.
Shweta Kalve   +4 more
doaj   +4 more sources

Hormone immunolabeling in resin-embedded Arabidopsis tissues

open access: yesSTAR Protocols, 2023
Here, we present a protocol for immunolabeling of molecules in Arabidopsis tissues. We describe steps for tissue fixation and embedding in resin of microtome-derived sections, immunolabeling using fluorescent and non-fluorescent secondary antibodies, and visualization of cytokinin and auxin molecules.
Humberto Herrera-Ubaldo   +4 more
openaire   +4 more sources

Lignans in Knotwood of Norway Spruce: Localisation with Soft X-ray Microscopy and Scanning Transmission Electron Microscopy with Energy Dispersive X-ray Spectroscopy

open access: yesMolecules, 2020
Lignans are bioactive compounds that are especially abundant in the Norway spruce (Picea abies L. Karst.) knotwood. By combining a variety of chromatographic, spectroscopic and imaging techniques, we were able to quantify, qualify and localise the easily
Tuomas Mansikkala   +11 more
doaj   +1 more source

A microwave method for plastic embedding of nervous tissue for light and electron microscopy

open access: yesHeliyon, 2020
Background: Fast, effective, and rapid processing of central nervous system (CNS) tissue with good preservation of myelin, especially in tissue from diseased mice, is important to many laboratories studying neurosciences.
Evan Calkins   +3 more
doaj   +1 more source

Simultaneous Acquisition of Multicolor Information From Neural Circuits in Resin-Embedded Samples

open access: yesFrontiers in Neuroscience, 2018
Resin embedding has been widely used for precise imaging of fluorescently labeled biological samples with optical and electron microscopy. The low preservation rate of fluorescence, especially for red fluorescent proteins, has limited the application of ...
Miao Ren   +14 more
doaj   +1 more source

Plastic Embedding and Sectioning of Plant Tissues

open access: yesBio-Protocol, 2012
Plastic (resin) embedding provides exclusively improvements to cellular definition compared to paraffin embedding. The combination of strongly cross-linking paraformaldehyde with glutaraldehyde and post fixed with OsO4 is the fixative of choice for high ...
Tie Liu
doaj   +1 more source

Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis

open access: yesMicromachines, 2020
Microfluidic devices utilizing spheroids play important roles in in vitro experimental systems to closely simulate morphological and biochemical characteristics of the in vivo tumor microenvironment.
Satoru Kuriu   +3 more
doaj   +1 more source

DNA from resin-embedded organisms: Past, present and future

open access: yesPLOS ONE, 2020
Past claims have been made for fossil DNA recovery from various organisms (bacteria, plants, insects and mammals, including humans) dating back in time from thousands to several million years BP. However, many of these recoveries, especially those described from million-year-old amber (fossil resin), have faced criticism as being the result of modern ...
David Peris   +8 more
openaire   +7 more sources

A novel 3D X‐ray computed tomography (CT) method for spatio‐temporal evaluation of waterlogging‐induced aerenchyma formation in barley

open access: yesPlant Phenome Journal, 2022
Increased precipitation during winter months in Western Europe is predicted from climate change. This is expected to increase the frequency of flooding events, with waterlogged conditions becoming more prevalent.
Stephen Kehoe   +5 more
doaj   +1 more source

Preparation and properties of a new type of flexible epoxy resin based on a molecular network structure

open access: yeseXPRESS Polymer Letters, 2021
In this paper, toluene-diisocyanate-trimer (TDI-T) was utilized to manufacture a new type of epoxy resin with high toughness via the co-polymerization method. In the procedure of preparing bisphenol A epoxy resin, before the reaction between bisphenol A (
X. F. Zhang   +4 more
doaj   +1 more source

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