Results 211 to 220 of about 226,996 (233)
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Restriction–modification system in bacteriophage MB78
Biochemical and Biophysical Research Communications, 2003Restriction-modification system is present in bacteria to protect the cells against phage infection. Interestingly, the bacteriophage MB78, a virulent phage of Salmonella typhimurium possesses restriction-modification system. Permissive host transformed with plasmid having the genomic fragment of MB78 carrying the putative restriction-modification ...
Deepti, Chaturvedi +1 more
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The restriction-modification system inStreptomyces flavopersicus
Folia Microbiologica, 2001To clone bifunctional vectors in streptomycetes, it was necessary to define the restriction-modification system of Streptomyces flavopersicus. Plasmid DNA from bifunctional vectors pIJ699 and pXED3-13, isolated from E. coli strains with different methylation systems: E. coli DH5 alpha (dam+ dcm+), E. coli MB5386 (dam dcm), E.
D, Lyutzkanova +2 more
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Cloned restriction-modification systems — a review
Gene, 1988The genes for numerous restriction endonucleases and modification methylases have been cloned into Escherichia coli. A summary is given for the clones isolated so far (115 entries) and of the procedures used to obtain them.
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Organization and sequence of the SalI restriction-modification system
Gene, 1994The organization and nucleotide (nt) sequences were determined for the genes encoding the SalI restriction and modification (R-M) system (recognition sequence 5'-GTCGAC-3') from Streptomyces albus G. The system comprises two genes, salIR, coding for the restriction endonuclease (ENase, R.SalI; probably 315 amino acids (aa), a predicted M(r) of 35,305 ...
M R, Rodicio +4 more
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Cloning of the HhaI and HinPI restriction-modification systems
Gene, 1988The genes for the HhaI (Roberts et al., 1976) and HinPI (Roberts, 1987) restriction-modification (R-M) systems have been cloned in pBR322. The HhaI system was isolated on a 9-kb PstI fragment, and the HinPI system was isolated on two PstI fragments of 1.5 and 4.6 kb in length.
J M, Barsomian, C O, Card, G G, Wilson
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Restriction-modification systems in Streptomyces antibioticus
Canadian Journal of Microbiology, 1985Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains.
J, Sànchez +4 more
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Type II restriction—modification systems
Trends in Genetics, 1988Abstract The genes for increasing numbers of type II restriction and modification enzymes are being cloned and characterized. The gene arrangements and enzyme sequences are quite diverse in spite of the biochemical similarities of the enzymes.
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Restriction-modification systems determined by Pseudomonas plasmids
Plasmid, 1982Abstract Four additional Pseudomonas R plasmids determining the Pae R7 restriction-modification system have been detected. All are transfer deficient and appear to belong to the same incompatibility group. The Pseudomonas fertility plasmid FP110 determines a different restriction-modification system and also inhibits the propagation of phage B39
G A, Jacoby, L, Sutton
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PHAGE-MEDIATED SELECTION AND THE EVOLUTION AND MAINTENANCE OF RESTRICTION-MODIFICATION
Evolution, 1993Restriction-modification (R-M) was discovered because it provides bacteria with immunity to phage infection. But, is phage-mediated selection the sole mechanism responsible for the evolution and maintenance of these ubiquitous and multiply evolved systems?
Ryszard, Korona, Bruce R, Levin
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Role of restriction-modification systems in prokaryotic evolution and ecology
Biochemistry (Moscow), 2015Restriction-modification (R-M) systems are able to methylate or cleave DNA depending on methylation status of their recognition site. It allows them to protect bacterial cells from invasion by foreign DNA. Comparative analysis of a large number of available bacterial genomes and methylomes clearly demonstrates that the role of R-M systems in bacteria ...
A S, Ershova +4 more
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