Results 41 to 50 of about 179,539 (265)

Evolutionary genome engineering using a restriction–modification system [PDF]

Nucleic Acids Research, 2011
Modification of complex microbial cellular processes is often necessary to obtain organisms with particularly favorable characteristics, but such experiments can take many generations to achieve. In the present article, we accelerated the experimental evolution of Escherichia coli populations under selection for improved growth using one of the ...
Asakura, Yoko, Kojima, Hiroyuki, Kobayashi, Ichizo   +2 more
openaire   +2 more sources

Organization of restriction-modification systems

Nucleic Acids Research, 1991
The genes for over 100 restriction-modification systems have now been cloned, and approximately one-half have been sequenced. Despite their similar function, they are exceedingly heterogeneous. The heterogeneity is evident at three levels: in the gene arrangements; in the enzyme compositions; and in the protein sequences. This paper summarizes the main
openaire   +3 more sources

Restriction–modification systems and bacteriophage invasion: Who wins? [PDF]

Journal of Theoretical Biology, 2010
The success of a phage that infects a bacterial cell possessing a restriction-modification (R-M) system depends on the activities of the host methyltransferase and restriction endonuclease, and the number of susceptible sites in the phage genome. However, there is no model describing this dependency and linking it to observable parameters such as the ...
Enikeeva, Farida N., Severinov, Konstantin V., Gelfand, Mikhail S.   +2 more
openaire   +2 more sources

Avoidance of recognition sites of restriction-modification systems is a widespread but not universal anti-restriction strategy of prokaryotic viruses

BMC Genomics, 2018
Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov, A. S. Ershova, A. S. Karyagina, S. A. Spirin, A. V. Alexeevski   +4 more
doaj   +1 more source

DNA restriction-modification systems mediate plasmid maintenance

Journal of Bacteriology, 1995
Two plasmid-carried restriction-modification (R-M) systems, EcoRI (from pMB1 of Escherichia coli) and Bsp6I (from pXH13 of Bacillus sp. strain RFL6), enhance plasmid segregational stability in E. coli and Bacillus subtilis, respectively. Inactivation of the endonuclease or the presence of the methylase in trans abolish the stabilizing activity of the R-
Kulakauskas, Saulius, S., Lubys, A., Ehrlich, S.D.   +2 more
openaire   +4 more sources

Bacterial Autoimmunity Due to a Restriction-Modification System [PDF]

Current Biology, 2016
Restriction-modification (RM) systems represent a minimal and ubiquitous biological system of self/non-self discrimination in prokaryotes [1], which protects hosts from exogenous DNA [2]. The mechanism is based on the balance between methyltransferase (M) and cognate restriction endonuclease (R).
Pleška, Maroš, Qian, Long, Okura, Reiko, Bergmiller, Tobias, Wakamoto, Yuichi, Kussell, Edo, Guet, Călin C.   +6 more
openaire   +2 more sources

A Quality Improvement Initiative to Standardize Pneumocystis jirovecii Pneumonia Prophylaxis in Pediatric Patients With Solid Tumors

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Pediatric patients with extracranial solid tumors (ST) receiving chemotherapy are at an increased risk for Pneumocystis jirovecii pneumonia (PJP). However, evidence guiding prophylaxis practices in this population is limited. A PJP‐related fatality at our institution highlighted inconsistent prescribing approaches and concerns about
Kriti Kumar, Denise M. Connolly, Ellen Kellington, Elyse Zelunka, Karla Kerrigan, Furqan Shaikh, Paul C. Nathan, Meredith S. Irwin, Sarah Cohen‐Gogo   +8 more
wiley   +1 more source

Engineering clostridium strain to accept unmethylated DNA. [PDF]

PLoS ONE, 2010
It is difficult to genetically manipulate the medically and biotechnologically important genus Clostridium due to the existence of the restriction and modification (RM) systems. We identified and engineered the RM system of a model clostridial species, C.
Hongjun Dong, Yanping Zhang, Zongjie Dai, Yin Li   +3 more
doaj   +1 more source

The Neisseria gonorrhoeae S.NgoVIII restriction/modification system: a type IIs system homologous to the Haemophilus parahaemolyticus HphI restriction/modification system [PDF]

Nucleic Acids Research, 1997
Strains of Neisseria gonorrhoeae possess numerous restriction-modification (R-M) systems. One of these systems, which has been found in all strains tested, encodes the S. NgoVIII specificity (5'TCACC 3') R-M system. We cloned two adjacent methyltransferase genes (dcmH and damH), each encoding proteins whose actions protect DNA from digestion by R.HphI ...
J S, Gunn, D C, Stein
openaire   +2 more sources

Psychosocial Outcomes in Patients With Endocrine Tumor Syndromes: A Systematic Review

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Introduction The combination of disease manifestations, the familial burden, and varying penetrance of endocrine tumor syndromes (ETSs) is unique. This review aimed to portray and summarize available data on psychosocial outcomes in patients with ETSs and explore gaps and opportunities for future research and care.
Daniël Zwerus, Floortje Strobbe, Annemarie A. Verrijn Stuart, Hanneke M. van Santen, Gerlof D. Valk, Sasja A. Schepers, Rachel S. van Leeuwaarde   +6 more
wiley   +1 more source