Results 21 to 30 of about 186,000 (245)
Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system
Microbial Biotechnology, 2023 Plasmid pUC11B is a 49.3‐kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01‐like conjugation system and tetracycline‐resistance.
Guillermo Ortiz Charneco +8 more
doaj +1 more source
Restriction-modification systems in Mycoplasma spp [PDF]
Genetics and Molecular Biology, 2007 Restriction and Modification (R-M) systems are present in all Mycoplasma species sequenced so far. The presence of these genes poses barriers to gene transfer and could protect the cell against phage infections. The number and types of R-M genes between different Mycoplasma species are variable, which is characteristic of a polymorphism.
Brocchi, Marcelo +2 more
openaire +5 more sources
Frontiers in Microbiology, 2021 Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek +6 more
doaj +1 more source
Transcription regulation of the EcoRV restriction-modification system [PDF]
Nucleic Acids Research, 2005 When a plasmid containing restriction-modification (R-M) genes enters a naïve host, unmodified host DNA can be destroyed by restriction endonuclease. Therefore, expression of R-M genes must be regulated to ensure that enough methyltransferase is produced and that host DNA is methylated before the endonuclease synthesis begins.
Semenova, Ekaterina +8 more
openaire +2 more sources
Frontiers in Microbiology, 2020 Campylobacter jejuni is a major cause of foodborne gastroenteritis worldwide inflicting palpable socioeconomic costs. The ability of this pathogen to successfully infect its hosts is determined not only by the presence of specific virulence genes but ...
Sandeep Ghatak +3 more
doaj +1 more source
A Type III restriction–modification system in Mycoplasma mycoides subsp. capri [PDF]
Open Biology, 2012 The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction–modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and ...
Mikkel A. Algire +4 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2022 Anti-restriction proteins are typically encoded by plasmids, conjugative transposons, or phages to improve their chances of entering a new bacterial host with a type I DNA restriction and modification (RM) system.
Huimin Chen +6 more
doaj +1 more source
BMC Microbiology, 2008 Background Many microbes possess restriction-modification systems that protect them from parasitic DNA molecules. Unfortunately, the presence of a restriction-modification system in a given microbe also hampers genetic analysis.
Ward Jordan D +3 more
doaj +1 more source
Cloning and Expression of the BalI Restriction-modification System [PDF]
Nucleic Acids Research, 1996 BalI, a type II restriction-modification (R-M) system from the bacterium, Brevibacterium albidum, recognizes the DNA sequence 5'-TGGCCA-3'. We cloned the genes encoding the BalI restriction endonuclease and methyltransferase and expressed them in Escherichia coli. The two genes were aligned tail-to-tail and their termination codons overlapped.
H, Ueno, I, Kato, Y, Ishino
openaire +2 more sources
Methylome-dependent transformation of emm1 group A streptococci
mBio, 2023 Genetic intractability presents a fundamental barrier to the manipulation of bacteria, hindering advancements in microbiological research. Group A Streptococcus (GAS), a lethal human pathogen currently associated with an unprecedented surge of infections
Joana Alves +4 more
doaj +1 more source