A mobile restriction modification system consisting of methylases on the IncA/C plasmid [PDF]
Mobile DNA, 2019 Background IncA/C plasmids play important roles in the development and dissemination of multidrug resistance in bacteria. These plasmids carry three methylase genes, two of which show cytosine specificity.
Ruibai Wang, Jing Lou, Jie Li
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From sequence to activity: the HgaI-homologous restriction modification system RM.MhoVI of Mycoplasma hominis [PDF]
BMC MicrobiologyRestriction-modification (RM) systems are widespread defense mechanisms in prokaryotes that protect the host from potentially harmful foreign DNA. They typically consist of a DNA methyltransferase (MTase), which methylates the host genome at an adenine ...
Lars Vogelgsang +5 more
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Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of
mBio, 2020 The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Claire Durmort +11 more
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Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg [PDF]
Journal of Biological EngineeringBackground Vibrio sp. dhg is a fast-growing, alginate-utilizing, marine bacterium being developed as a platform host for macroalgae biorefinery. To maximize its potential in the production of various value-added products, there is a need to expand ...
Yang Jun Shon +5 more
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Effects of Population Dynamics on Establishment of a Restriction-Modification System in a Bacterial Host [PDF]
Molecules, 2019 In vivo dynamics of protein levels in bacterial cells depend on both intracellular regulation and relevant population dynamics. Such population dynamics effects, e.g., interplay between cell and plasmid division rates, are, however, often neglected in ...
Stefan Graovac +4 more
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Low-level expression of the Type II restriction-modification system confers potent bacteriophage resistance in Escherichia coli. [PDF]
DNA Res, 2020 Wilkowska K +3 more
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mSystems, 2023 With the advance of high-throughput sequencing, the molecular basis of coevolutionary interaction between viruses and host microorganisms is predominantly elucidated by in silico genomic analyses, which revealed potential communication of genetic ...
Yi Zhang +6 more
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Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes of the EcoVIII Restriction-Modification System, Is Mobilizable among Enterobacteria. [PDF]
PLoS ONE, 2016 Type II restriction-modification systems are ubiquitous in prokaryotes. Some of them are present in naturally occurring plasmids, which may facilitate the spread of these systems in bacterial populations by horizontal gene transfer.
Olesia Werbowy, Tadeusz Kaczorowski
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Restriction modification systems as engines of diversity [PDF]
Frontiers in Microbiology, 2015 Restriction modification (RM) systems provide protection against a broad spectrum of phages. However, the likelihood of a phage permanently bypassing this can be as high as 0.1 per infection (Korona et al., 1993) which makes for a relatively weak defense.
Sneppen, Kim +3 more
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PLoS Genetics, 2022 Understanding mechanisms that shape horizontal exchange in prokaryotes is a key problem in biology. A major limit on DNA entry is imposed by restriction-modification (RM) processes that depend on the pattern of DNA modification at host-specified sites ...
Julie Zaworski +6 more
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