Results 121 to 130 of about 10,132,911 (226)

Comparative and functional genomic analysis of foreign DNA defense mechanisms in Enterococcus faecium

Microbiology Spectrum
Enterococcus faecium is notoriously difficult to study genetically due to the poor understanding of barriers preventing foreign DNA uptake, such as the proteins that modify type I restriction modification (RM) system activity.
Alexandra L. Krause, Louise M. Judd, Ryan Wick, Timothy P. Stinear, Andrew H. Buultjens, Ian R. Monk   +5 more
doaj   +1 more source

A sequential one-pot approach for rapid and convenient characterization of putative restriction-modification systems. [PDF]

mSystems, 2023
Zhang Y, Takaki Y, Yoshida-Takashima Y, Hiraoka S, Kurosawa K, Nunoura T, Takai K.   +6 more
europepmc   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Restriction-modification systems have shaped the evolution and distribution of plasmids across bacteria. [PDF]

Nucleic Acids Res, 2023
Shaw LP, Rocha EPC, MacLean RC.
europepmc   +1 more source

Modeling and bioinformatics of bacterial immune systems: understanding regulation of CRISPR/Cas and restriction-modification systems

, 2017
Summary. Bacterial immune systems protect bacterial cells from foreign DNA, such as viruses and plasmids. They also critically affect bacterial pathogenicity by reducing the flow of genes between bacteria.
Guzina, Jelena, Blagojević, Bojana, Đorđević, Marko, Rodić, Anđela   +3 more
core   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus

Microbial Cell Factories
Background To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize transformation methods.
Zhao Xingya, Fu Xiaoping, Zhen Jie, Yang Jun, Zheng Hongchen, Bai Wenqin, Song Hui   +6 more
doaj   +1 more source

Bacterial autoimmunity due to a restriction-modification system

, 2016
Restriction-modification (RM) systems represent a minimal and ubiquitous biological system of self/non-self discrimination in prokaryotes [1], which protects hosts from exogenous DNA [2]. The mechanism is based on the balance between methyltransferase (M)
Bergmiller, Tobias ; https://orcid.org/, Wakamoto, Yuichi, Guet, Calin C ; https://orcid.org/, Kussell, Edo, Okura, Reiko, Pleska, Maros ; https://orcid.org/, Qian, Long   +6 more
core   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source