Results 261 to 270 of about 728,418 (291)

Cloned restriction-modification systems — a review

Gene, 1988
The genes for numerous restriction endonucleases and modification methylases have been cloned into Escherichia coli. A summary is given for the clones isolated so far (115 entries) and of the procedures used to obtain them.
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Organization and sequence of the SalI restriction-modification system

Gene, 1994
The organization and nucleotide (nt) sequences were determined for the genes encoding the SalI restriction and modification (R-M) system (recognition sequence 5'-GTCGAC-3') from Streptomyces albus G. The system comprises two genes, salIR, coding for the restriction endonuclease (ENase, R.SalI; probably 315 amino acids (aa), a predicted M(r) of 35,305 ...
M R, Rodicio, T, Quinton-Jager, L S, Moran, B E, Slatko, G G, Wilson   +4 more
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Cloning of the HhaI and HinPI restriction-modification systems

Gene, 1988
The genes for the HhaI (Roberts et al., 1976) and HinPI (Roberts, 1987) restriction-modification (R-M) systems have been cloned in pBR322. The HhaI system was isolated on a 9-kb PstI fragment, and the HinPI system was isolated on two PstI fragments of 1.5 and 4.6 kb in length.
J M, Barsomian, C O, Card, G G, Wilson
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Restriction-modification systems in Streptomyces antibioticus

Canadian Journal of Microbiology, 1985
Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains.
J, Sànchez, C, Barbès, A, Hernandez, C R, de los Reyes Gavilàn, C, Hardisson   +4 more
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Type II restriction—modification systems

Trends in Genetics, 1988
Abstract The genes for increasing numbers of type II restriction and modification enzymes are being cloned and characterized. The gene arrangements and enzyme sequences are quite diverse in spite of the biochemical similarities of the enzymes.
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Restriction-modification systems determined by Pseudomonas plasmids

Plasmid, 1982
Abstract Four additional Pseudomonas R plasmids determining the Pae R7 restriction-modification system have been detected. All are transfer deficient and appear to belong to the same incompatibility group. The Pseudomonas fertility plasmid FP110 determines a different restriction-modification system and also inhibits the propagation of phage B39
G A, Jacoby, L, Sutton
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Cloning the KpnI restriction-modification system in Escherichia coli

Gene, 1991
The genes encoding the KpnI restriction and modification (R-M) system from Klebsiella pneumoniae, recognizing the sequence, 5'-GGTAC decreases C-3', were cloned and expressed in Escherichia coli. Although the restriction endonuclease (ENase)- and methyltransferase (MTase)-encoding genes were closely linked, initial attempts to clone both genes as a ...
A W, Hammond, G F, Gerard, D K, Chatterjee   +2 more
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Cloning and characterization of the genes of the CeqI restriction—modification system

The International Journal of Biochemistry & Cell Biology, 1997
Two genes from Corynebacterium equii, a Gram-positive bacterium producing the CeqI restriction-modification enzymes were cloned and sequenced. In vivo restriction experiments, DNA and amino acid sequence data suggest that the two genes code for the endonuclease and the methyltransferase enzymes. However, when the two genes are expressed in E.
Z, Izsvák, Z, Jobbágy, I, Takács, E, Duda   +3 more
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GATC-specific restriction-modification systems in ruminal bacteria

Folia Microbiologica, 2004
The GATC-specific restriction and modification activities were analyzed in 11 major bacterial representatives of ruminal microflora. Modification phenotype was observed in 13 out of 40 ruminal strains. MboI isoschizomeric restriction endonucleases were detected in 10 bacterial strains tested; three strains lacked any detectable corresponding ...
M, Piknová, P, Pristas, P, Javorský
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Polymorphism of Bacterial Restriction-Modification Systems: The Advantage of Diversity

Evolution, 1994
Bacterial restriction-modification systems provide defense against foreign DNA by using a self versus nonself recognition mechanism. A great diversity of recognition motifs is maintained in natural populations. Circumstantial evidence suggests that defense against bacteriophage viruses favors this diversity.
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