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Characterization of AloI, a restriction-modification system of a new type

Journal of Molecular Biology, 2001
We report the properties of the new AloI restriction and modification enzyme from Acinetobacter lwoffi Ks 4-8 that recognizes the DNA target 5' GGA(N)6GTTC3' (complementary strand 5' GAAC(N)6TCC3'), and the nucleotide sequence of the gene encoding this enzyme.
E, Cesnaviciene   +6 more
openaire   +2 more sources

Comparison of the Homologous SfeI and LlaBI Restriction–Modification Systems

Molecular Biology, 2002
A fragment containing the SfeI restriction-modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris W56 LlaBI RMS recognizing the same site, 5'-CTRYAG-3'.
S S, Okhapkina   +6 more
openaire   +2 more sources

Cloning the BstVI restriction-modification system in Escherichia coli

Gene, 1991
A standard DNA modification methyltransferase (MTase) selection protocol was followed to clone the BstVI restriction and modification system from Bacillus stearothermophilus in Escherichia coli. Both genes were contained in a 4.4-kb EcoRI fragment from B. stearothermophilus V chromosomal DNA. The heterologous expression of these genes did not depend on
C, Vásquez, C, Saavedra, E, González
openaire   +2 more sources

Evolution of restriction-modification systems

2018
Restriction Modification (R-M) systems prevent the invasion of foreign genetic material into bacterial cells and are therefore important in maintaining the integrity of the host genome. The spread of antibiotic resistance, which is proposed to occur via the transfer of foreign genes to the bacterial genome, makes the subject of R-M systems extremely ...
openaire   +1 more source

The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex

Nature Catalysis, 2022
Dan Wu   +10 more
semanticscholar   +1 more source

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