Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases
FEBS Letters, EarlyView.The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher +6 more
wiley +1 more source
Tranformasi Fragmen Dna Kromosom Xanthomonas Campestris ke dalam Escherichia Coli
Makara Seri Sains, 2002 Research on DNA transformation of Xanthomonas campestris into Escherichia coli DH5αα using plasmid vector Escherichia coli (pUC19). was carried out. DNA chromosome was isolated using CTAB method, alkali lysis method was used to isolate DNA plasmid.
Wibowo Mangunwardoyo
doaj
Genotyping and antimicrobial resistance patterns of Klebsiella pneumoniae clinical isolates in Suez Canal University Hospitals [PDF]
Microbes and Infectious DiseasesBackground: Health crisis of multi-drug resistant Klebsiella pneumoniae (K. pneumoniae) seems overwhelming. Genotyping for such an important pathogen is a demand to control aggressive Klebsiella infections. Therefore, this study was conducted to identify
Maha Mahdi +4 more
doaj +1 more source
A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis [PDF]
, 2007 Background The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production.
Koch, Jørn +2 more
core +3 more sources
Journal of Clinical Microbiology, 2006 Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s.
V. Rodriguez-Nava +5 more
semanticscholar +1 more source
Insertion of the FeB cofactor in cNORs lacking metal inserting chaperones
FEBS Letters, EarlyView.Nitric oxide reductase is an enzyme found in the bacterial denitrification pathway. The NOR active site contains a non‐heme iron, often, but not always inserted with the assistance of chaperones. Here, we study the insertion of FeB in the subfamily of cNORs lacking chaperones and found a putative channel, conserved in the family, perhaps enabling the ...
Sofia Appelgren, Pia Ädelroth
wiley +1 more source
Activity on non-methylated DNA limits the use of endonuclease MspJI for epigenetic analyses
All Results Journals: Biol, 2018 Cytosine methylation of DNA in mammals has been associated with both physiological and pathological changes in gene-expression. DNA treatment with methylation sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used ...
María Belén Jerez +1 more
doaj
Computation of splicing languages from DNA splicing system with one palindromic restriction enzyme [PDF]
, 2018 In DNA splicing system, the potential effects of sets of restriction enzymes and a ligase that allow DNA molecules to be cleaved and reassociated to produce further molecules are studied.
Fong, Wan Heng +2 more
core +1 more source
FEBS Letters, EarlyView.LHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Victor Zegarra +7 more
wiley +1 more source
The Efficiency of Transgenesis by Restriction Enzyme Mediated Integration - Sperm Mediated Gene Transfer (REMI-SMGT) [PDF]
, 2013 The present study tends to test the validity of REMI-SMGT and to evaluate the efficiency of REMI-SMGT on generating other mammalian species rather than the sheep that made only by one group and to observe the possibility of doing so by using rabbits as ...
Al-Saadi, Ali +3 more
core +1 more source