Results 101 to 110 of about 2,983,763 (344)

A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

BMC Biotechnology, 2007
Background The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production.
Stougaard Magnus, Lohmann Jakob S, Koch Jørn   +2 more
doaj   +1 more source

PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism

Molecular Oncology, EarlyView.
This study investigated how PYCR1 inhibition in bone marrow stromal cells (BMSCs) indirectly affects multiple myeloma (MM) cell metabolism and viability. Culturing MM cells in conditioned medium from PYCR1‐silenced BMSCs impaired oxidative phosphorylation and increased sensitivity to bortezomib.
Inge Oudaert, Lauren van den Broecke, Osman Aksoy, Judith Lind, Sonia Vallet, Arne Van der Vreken, Gamze Ates, Ann Massie, Ken Maes, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Klaus Podar, Eline Menu   +13 more
wiley   +1 more source

Patient‐specific pharmacogenomics demonstrates xCT as predictive therapeutic target in colon cancer with possible implications in tumor connectivity

Molecular Oncology, EarlyView.
This study integrates transcriptomic profiling of matched tumor and healthy tissues from 32 colorectal cancer patients with functional validation in patient‐derived organoids, revealing dysregulated metabolic programs driven by overexpressed xCT (SLC7A11) and SLC3A2, identifying an oncogenic cystine/glutamate transporter signature linked to ...
Marco Strecker, Keren Zohar, Martin Böttcher, Thomas Wartmann, Henry Freudenstein, Maximilian Doelling, Mihailo Andric, Wenjie Shi, Or Kakhlon, Katrin Hippe, Beatrix Jahnke, Dimitrios Mougiakakos, Franziska Baenke, Daniel Stange, Roland S. Croner, Michal Linial, Ulf D. Kahlert   +16 more
wiley   +1 more source

Association Analysis of miR423 (rs6505162) and miR608 (rs4919510) Polymorphisms with Lung Cancer Risk [PDF]

Journal of Mazandaran University of Medical Sciences
Background and purpose: Lung cancer is one of the deadliest cancers in the world, early detection of lung cancer is one of the biggest challenges in treating this disease and can be very effective in saving patients.
Javad Salehi, Morteza Sadeghi
doaj  

The type IIS restriction enzyme MmeI can cut across a double-strand break. [PDF]

Mol Biol Rep, 2023
Tasnim M, Selph TJ, Olcott J, Hill JT.
europepmc   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Conversion of barley SNPs into PCR-based markers using dCAPS method

Genetics and Molecular Biology, 2009
Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome.
Fahimeh Shahinnia, Badraldin Ebrahim Sayed-Tabatabaei   +1 more
doaj  

Characterization of a restriction enzyme from a strain ofNeisseria gonorrhoeawhich recognizes 5′GICCGGC3′, an isochizomer ofNael [PDF]

, 1989
Alan W. Hammond, Gary F. Gerard, James H. Campbell, Deb K. Chatterjee   +3 more
openalex   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

RESTRICTION ENDONUCLEASE ANALYSIS OF PASTEURELLA MULTOCIDA FIELD ISOLATES BY Hha-I [PDF]

Pakistan Veterinary Journal, 2006
In this study the restriction endonuclease analysis (REA) technique was used for the comparison of field isolates and vaccinal strain of Pasteurella multocida using Hha-I enzyme.
R. MUNIR, U. FAROOQ, N. BADAR, A. KHANUM1 AND A. WADOOD1
doaj