Results 101 to 110 of about 647,244 (359)

Restriction landmark genomic scanning (RLGS) spot identification by second generation virtual RLGS in multiple genomes with multiple enzyme combinations. [PDF]

, 2007
BackgroundRestriction landmark genomic scanning (RLGS) is one of the most successfully applied methods for the identification of aberrant CpG island hypermethylation in cancer, as well as the identification of tissue specific methylation of CpG islands ...
Ansari, Tahmina, Camoriano, Marta, Chen, Shih-Shih, Costello, Joseph, Dai, Zunyan, Dawson, David W, Elliott, Rosemary, Held, William, Hong, Jason S, Ioshikhes, Ilya, Kazhiyur-Mannar, Ramakrishnan, Liang, Ping, Liu, Chunhui, Oakes, Christopher C, Plass, Christoph, Rush, Laura J, Smiraglia, Dominic J, Smith, Laura T, Song, Fei, Su, Jian, Szafranek, Angela, Teitell, Michael A, Trasler, Jacquetta M, Wang, Shu-Huei, Wenger, Rephael, Wu, Yue-Zhong, Yu, Li   +26 more
core   +3 more sources

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Primary Structure and Catalytic Mechanism of the Epoxide Hydrolase from Agrobacterium radiobacter AD1 [PDF]

, 1997
The epoxide hydrolase gene from Agrobacterium radiobacter AD1, a bacterium that is able to grow on epichlorohydrin as the sole carbon source, was cloned by means of the polymerase chain reaction with two degenerate primers based on the N-terminal and C ...
Altschul, Andrade, Arand, Arand, Armstrong, Ausubel, Beetham, Beetham, Bell, Burley, Dick B. Janssen, Dı́az, Falany, Fujio, Geourjon, Grant, Hassett, Hecht, Heidelbach, Innis, Itzhaki, Jacobs, Janssen, Janssen, Johnson, Kawasaki, Kiyosue, Knehr, Kunkel, Lacourciere, Loewenthal, Marko Fennema, Mehta, Miller, Minke Smids, Noble, Ollis, Pries, Provencher, Rick Rink, Rost, Sambrook, Sanger, Schanstra, Skoda, Solovyev, Sreerama, Stapleton, Studier, Thomas, Thompson, Uwe Dehmel, Van den Wijngaard, Verschueren, Verschueren, Wang, Wilson, Winkler, Wojtasek, Yanisch-Perron   +59 more
core   +3 more sources

SNP-RFLPing: restriction enzyme mining for SNPs in genomes

BMC Genomics, 2006
Background The restriction fragment length polymorphism (RFLP) is a common laboratory method for the genotyping of single nucleotide polymorphisms (SNPs).
Cheng Yu-Huei, Chang Phei-Lang, Yang Cheng-Hong, Chang Hsueh-Wei, Chuang Li-Yeh   +4 more
doaj   +1 more source

The protein cost of metabolic fluxes: prediction from enzymatic rate laws and cost minimization [PDF]

, 2016
Bacterial growth depends crucially on metabolic fluxes, which are limited by the cell's capacity to maintain metabolic enzymes. The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering.
arxiv   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Subtracted Restriction Fingerprinting—A Tool for Bacterial Genome Typing

BioTechniques, 2003
Reproducible, discriminative, high-throughput methods are required for the identification of bacterial strains and isolates in a clinical environment. A new molecular typing method for bacteria was developed and tested on Salmonella and E.
Valeri Terletski, Stefan Schwarz, Joseph Carnwath, Heiner Niemann   +3 more
doaj   +1 more source

Kinetics of target site localization of a protein on DNA: a stochastic approach [PDF]

Biophys. J., September 1, 2004; 87(3): 1640 - 1649, 2005
It is widely recognized that the cleaving rate of a restriction enzyme on target DNA sequences is several orders of magnitude faster than the maximal one calculated from the diffusion--limited theory. It was therefore commonly assumed that the target site interaction of a restriction enzyme with DNA has to occur via two steps: one--dimensional ...
arxiv  

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

A cleavage map of bacteriophage Phi-X174 genome [PDF]

, 1974
Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments.
Lee, Amy Shiu, Sinsheimer, Robert L.
core