Results 101 to 110 of about 3,197,803 (398)

Using CRISPR-Cas9 as a Restriction Enzyme [PDF]

, 2020
Restriction digests are a commonly utilized process for cleaving DNA at specific, but relatively common sites. Restriction enzymes have widespread use in DNA manipulation.
Crawley, Zack
core   +1 more source

Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells

FEBS Letters, EarlyView.
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi   +6 more
wiley   +1 more source

Generating site saturation mutagenesis libraries and transferring them to broad host range plasmids using type IIS restriction enzymes [PDF]

arXiv, 2023
Protein engineering is an established method for tailoring enzymatic reactivity. A commonly used method is directed evolution, where the mutagenesis and natural selection process is mimicked and accelerated in the laboratory. Here, we describe a reliable method for generating saturation mutagenesis libraries by golden gate cloning in a broad host range
arxiv  

Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease.

Proceedings of the National Academy of Sciences of the United States of America, 2005
Among all restriction endonucleases known to date, BfiI is unique in cleaving DNA in the absence of metal ions. BfiI represents a different evolutionary lineage of restriction enzymes, as shown by its crystal structure at 1.9-A resolution.
S. Gražulis, E. Manakova, M. Roessle, M. Bochtler, G. Tamulaitienė, R. Huber, V. Šikšnys   +6 more
semanticscholar   +1 more source

The multiple roles of the NlpC_P60 peptidase family in mycobacteria – an underexplored target for antimicrobial drug discovery

FEBS Letters, EarlyView.
The NlpC_P60 superfamily of peptidases is recognised by its key role in bacterial cell wall homeostasis. Recently, studies have also described the involvement of NlpC_P60‐like enzymes in bacterial competitive mechanisms and pathogenesis across several lineages.
Catharina dos Santos Silva, Marcio Vinicius Bertacine Dias   +1 more
wiley   +1 more source

Possibilities of using nested PCR-RFLP for taxonomic identification of L3 larvae of the family Trichostrongylidae, Leiper, 1912

Российский паразитологический журнал
The purpose of the research is to apply molecular genetic research methods to identify the taxonomic affiliation of gastrointestinal parasitic sheep nematodes of the family Trichostrongylidae using nested PCR followed by the restriction fragment length ...
I. A. Pimenov, I. M. Odoevskaya, A. M. Plieva, A. I. Varlamova   +3 more
doaj   +1 more source

A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus. [PDF]

PLoS ONE, 2014
PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion.
Maria W Smith, Andrei L Ghindilis, Ihab A Seoudi, Kenneth Smith, Rosalind Billharz, Holly M Simon   +5 more
doaj   +1 more source

Single-Molecule Morphology of Topologically Digested Olympic Networks [PDF]

arXiv, 2023
The kinetoplast DNA (kDNA) is the archetype of a two-dimensional Olympic network, composed of thousands of DNA minicircles and found in the mitochondrion of certain parasites. The evolution, replication and self-assembly of this structure are fascinating open questions in biology that can also inform us how to realise synthetic Olympic networks in ...
arxiv  

Site-specific DNA transesterification catalyzed by a restriction enzyme

Proceedings of the National Academy of Sciences of the United States of America, 2007
Most restriction endonucleases use Mg2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification ...
G. Sasnauskas, B. Connolly, S. Halford, V. Šikšnys   +3 more
semanticscholar   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source