Results 101 to 110 of about 2,927,400 (332)

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2) [PDF]

Iranian Journal of Parasitology, 2011
Background: In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species.Methods: The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species.
M Forouzandeh-Moghadam, MB Rokni, A Dalimi, M Mahami-Oskouei   +3 more
doaj   +2 more sources

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source

Restriction Enzyme Analysis

Definitions, 2020

semanticscholar   +1 more source

A general method for the purification of restriction enzymes

Nucleic Acids Research, 1978
An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
Francisco Bolívar, Raymond L. Rodriguez, David J. Russel, Robert C. Tait, Herbert W. Boyer, Alejandra A. Covarrubias, Herbert L. Heyneker, Mary C. Betlach, Patricia J. Greene, Keith Backman   +9 more
openaire   +4 more sources

Genotypic and Serotypic confirmations of Bacterial community to Kotumsar cave for occupational safety of cave workers and visitors from pathogenic threats

International Journal of Occupational Safety and Health, 2017
Background: Bacterial communities exist everywhere in the universe so in the caves. Kotumsar cave is the one of the renowned tourist place in India therefore high risk of anthropogenic pressure in this ecological niche.
Yogita Rajput, Arvind Neral, Vibhuti Rai
doaj  

SNP-RFLPing 2: an updated and integrated PCR-RFLP tool for SNP genotyping

BMC Bioinformatics, 2010
Background PCR-restriction fragment length polymorphism (RFLP) assay is a cost-effective method for SNP genotyping and mutation detection, but the manual mining for restriction enzyme sites is challenging and cumbersome.
Chang Hsueh-Wei, Cheng Yu-Huei, Chuang Li-Yeh, Yang Cheng-Hong   +3 more
doaj   +1 more source

Cleavage of Simian Virus 40 DNA at a Unique Site by a Bacterial Restriction Enzyme [PDF]

, 1972
John F. Morrow, Paul Berg
openalex   +1 more source

Targeting the AKT/mTOR pathway attenuates the metastatic potential of colorectal carcinoma circulating tumor cells in a murine xenotransplantation model

Molecular Oncology, EarlyView.
Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit, Thais Pereira‐Veiga, Helena Brauer, Michael Horn, Paula Nissen, Thomas Mair, Bente Siebels, Hannah Voß, Ruimeng Zhuang, Marie‐Therese Haider, Desiree Loreth, Margarita Iskhakova, Bele Lindemann, Julian Kött, Laure Cayrefourcq, Jasmin Wellbrock, Hartmut Schlüter, Klaus Pantel, Catherine Alix‐Panabières, Manfred Jücker   +19 more
wiley   +1 more source

Isolation of large molecular weight DNA from agarose gels forfurther digestion by restriction enzymes [PDF]

, 1975
Nikolaus Blin, Alexander von Gabain, Hermann Bujard   +2 more
openalex   +1 more source