Results 141 to 150 of about 3,197,803 (398)

Integrative systems‐level analysis reveals a contextual crosstalk between hypoxia and global metabolism in human breast tumors

Molecular Oncology, EarlyView.
Breast tumor samples scored for metabolic deregulation (M1 to M3) were given a hypoxia score (HS). The highest HS occurred in patients with strongest metabolic deregulation (M3), supporting tumor aggressiveness. HS correlated with the highest number of metabolic pathways in M1. This suggests hypoxia to be an early event in metabolic deregulation.
Raefa Abou Khouzam, Salem Chouaib, Mohammad Askandar Iqbal   +2 more
wiley   +1 more source

PCR-RFLP of mitochondrial DNA of swamp buffaloes in breeding station

Italian Journal of Animal Science, 2010
Methods have been devised for detecting polymorphisms in the D-loop regions using the PCR followed by restriction enzyme digestion to reveal restriction fragment length polymorphism.
M. Kamonpatana, S. Usawang, W. Nualchuen, K. Tasripoo, S. Chethasing, C. Pansin, K. Srisakwattana   +6 more
doaj   +1 more source

Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5′-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi [PDF]

, 2005
To search for restriction endonucleases, we used a novel plant-based cell-free translation procedure that bypasses the toxicity of these enzymes. To identify candidate genes, the related genomes of the hyperthermophilic archaea Pyrococcus abyssi and ...
Bujnicki, Janusz M., Ishikawa, Ken, Kawakami, Bunsei, Kobayashi, Ichizo, Kuroita, Toshihiro, Tanokura, Masaru, Uchiyama, Ikuo, Watanabe, Miki   +7 more
core   +2 more sources

AAL-Toxin-Deficient Mutants of Alternaria alternata Tomato Pathotype by Restriction Enzyme-Mediated Integration.

Phytopathology, 1997
Host-specific toxins are produced by three pathotypes of Alternaria alternata: AM-toxin, which affects apple; AK-toxin, which affects Japanese pear; and AAL-toxin, which affects tomato. Each toxin has a role in pathogenesis.
H. Akamatsu, Y. Itoh, M. Kodama, H. Otani, K. Kohmoto   +4 more
semanticscholar   +1 more source

Detection rate for ESR1 mutations is higher in circulating‐tumor‐cell‐derived genomic DNA than in paired plasma cell‐free DNA samples as revealed by ddPCR

Molecular Oncology, EarlyView.
Analysis of ESR1 mutations in plasma cell‐free DNA (cfDNA) is highly important for the selection of treatment in patients with breast cancer. Using multiplex‐ddPCR and identical blood draws, we investigated whether circulating tumor cells (CTCs) and cfDNA provide similar or complementary information for ESR1 mutations.
Stavroula Smilkou, Aliki Ntzifa, Victoria Tserpeli, Ioanna Balgkouranidou, Alkistis Papatheodoridi, Evangelia Razis, Helena Linardou, Christos Papadimitriou, Amanda Psyrri, Flora Zagouri, Stylianos Kakolyris, Evi Lianidou   +11 more
wiley   +1 more source

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro [PDF]

, 2010
Plasmids, conjugative transposons and phage frequently encode anti-restriction proteins to enhance their chances of entering a new bacterial host that is highly likely to contain a Type I DNA restriction and modification (RM) system.
Adamczyk, Alexandrov, Altschul, Andrew P. Herbert, Atanasiu, Bairoch, Bandyopadhyay, Belogurov, Belogurov, Belogurov, Bennett-Lovsey, Berman, Blakely, Bond, Brunger, Burlage, Cazalet, Combet, Cornilescu, David T. F. Dryden, Dimitra Serfiotis-Mitsa, Dinesh C. Soares, Dosset, Dryden, Dunn, Dušan Uhrín, Fraczkiewicz, Frishman, Gareth A. Roberts, Garry W. Blakely, Gill, Glaser, Grzesiek, Guntert, Harada, Heiden, Herrmann, Holm, Hutchinson, Hutchinson, John H. White, Kamachi, Kay, Kennaway, Koradi, Krissinel, Kumar, Larsen, Laskowski, Loenen, Makovets, Makovets, McMahon, Muhandiram, Murray, Nekrasov, Nicholls, Ottiger, Pei, Rasko, Riley, Roberts, Saltman, Serfiotis-Mitsa, Sklenar, Stephanou, Stephanou, Studier, Thomas, Thomas, Tjandra, Tock, Uhrinova, Ulrich, Valafar, Vedler, Vranken, Vriend, Walkinshaw, Webb, Welch, Wilkins, Yamazaki, Yip, Zavilgelsky, Zavilgelsky   +85 more
core   +2 more sources

Optimal enzyme rhythms in cells [PDF]

arXiv, 2016
Cells can use periodic enzyme activities to adapt to periodic environments or existing internal rhythms and to establish metabolic cycles that schedule biochemical processes in time. A periodically changing allocation of the protein budget between reactions or pathways may increase the overall metabolic efficiency.
arxiv  

Identification of various medically important Candida species in clinical specimens by PCR-restriction enzyme analysis

Journal of Clinical Microbiology, 1997
A single primer pair amplifying a cytochrome P-450 lanosterol-14 alpha-demethylase (L1A1) gene fragment that encodes a highly conserved region was used to detect yeast DNA in clinical specimens.
G. Morace, M. Sanguinetti, B. Posteraro, G. Lo Cascio, G. Fadda   +4 more
semanticscholar   +1 more source

Targeted metabolomics reveals novel diagnostic biomarkers for colorectal cancer

Molecular Oncology, EarlyView.
This study employed targeted metabolomic profiling to identify 302 distinct metabolites present in platelet‐rich plasma (PRP), revealing aberrant metabolic profiles amongst individuals diagnosed with colorectal cancer (CRC). Compared to carcinoembryonic antigen (CEA) and cancer antigen 19‐9 (CA199), our metabolite panel showed improved sensitivity ...
Zuojian Hu, Fenglin Shen, Yang Liu, Ziqing Zhong, Yongling Chen, Zhiyuan Xia, Cuiju Mo, Hongxiu Yu   +7 more
wiley   +1 more source

Bioinformatic Analysis Strategy in Restriction Enzyme Selection for Indonesian Panulirus homarus Identification by PCR-RFLP [PDF]

The spiny lobster (Panulirus homarus) is a valuable fishery commodity in Indonesia, particularly in its juvenile life form. However, identifying the early life forms of the spiny lobster can be challenging, as it exhibits similar morphological features ...
Indriatmoko, Indriatmoko, Pancoro, Adi
core   +4 more sources