Comparison of Mycoplasma arthritidis strains by enzyme-linked immunosorbent assay, immunoblotting, and DNA restriction analysis [PDF]
, 1995 L R Washburn +6 more
openalex +1 more source
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Expanding the scope of methylation-sensitive restriction enzyme (MSRE) PCR for forensic identification of body fluids through the novel use of methylation-dependent restriction enzymes (MDRE) and the combination of autosomal and Y-chromosomal markers. [PDF]
Int J Legal MedRothe J +6 more
europepmc +1 more source
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. [PDF]
, 1996 Y G Kim +2 more
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Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
UbaLAI is a monomeric Type IIE restriction enzyme. [PDF]
Nucleic Acids Res, 2017 Sasnauskas G +7 more
europepmc +1 more source
The restriction enzymeAIwNI is blocked by overlapping methylation
, 1991 Martin Bourbonnière +1 more
openalex +2 more sources
Improved Method for Genotyping Apolipoprotein E Polymorphisms by a PCR-Based Assay Simultaneously Utilizing Two Distinct Restriction Enzymes [PDF]
, 1997 Ariella Zivelin +5 more
openalex +1 more source