Results 231 to 240 of about 2,983,763 (344)

Designing Randomized DNA Sequences Free of Restriction Enzyme Recognition Sites. [PDF]

Biotechnol J, 2018
Storm AJ, Jensen PA.
europepmc   +1 more source

Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.

Nucleic Acids Research, 1999
D. Tillett, B. Neilan
semanticscholar   +1 more source

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

DNA supercoiling enables the Type IIS restriction enzyme BspMI to recognise the relative orientation of two DNA sequences

, 2003
Isabel J. Kingston
openalex   +1 more source

PCR-Restriction Enzyme Analysis for Detection ofCandida DNA in Blood from Febrile Patients with Hematological Malignancies

Journal of Clinical Microbiology, 1999
G. Morace, L. Pagano, M. Sanguinetti, B. Posteraro, L. Mele, F. Equitani, G. D’Amore, G. Leone, G. Fadda   +8 more
semanticscholar   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

msgbsR: An R package for analysing methylation-sensitive restriction enzyme sequencing data. [PDF]

Sci Rep, 2018
Mayne BT, Leemaqz SY, Buckberry S, Rodriguez Lopez CM, Roberts CT, Bianco-Miotto T, Breen J.   +6 more
europepmc   +1 more source

The role of lipid metabolism in neuronal senescence

FEBS Open Bio, EarlyView.
Disrupted lipid metabolism, through alterations in lipid species or lipid droplet accumulation, can drive neuronal senescence. However, lipid dyshomeostasis can also occur alongside neuronal senescence, further amplifying tissue damage. Delineating how lipid‐induced senescence emerges in neurons and glial cells, and how it contributes to ageing and ...
Dikaia Tsagkari, Eleftheria Panagiotidou, Nektarios Tavernarakis   +2 more
wiley   +1 more source

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion. [PDF]

J Vis Exp, 2017
Santillán O, Ramírez-Romero MA, Dávila G.   +2 more
europepmc   +1 more source