Results 251 to 260 of about 620,807 (343)

MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. [PDF]

Nucleic Acids Res, 2018
Lin D, O'Callaghan CA.
europepmc   +1 more source

219 Effects of maternal nutrient restriction and rumen-protected arginine supplementation on post-ruminal digestive enzyme activities of lamb offspring

, 2020
Ronald J Trotta, Faithe Keomanivong, Jena Lee Bjertness, Joel S Caton, Kendall C Swanson   +4 more
openalex   +2 more sources

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

FEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

msgbsR: An R package for analysing methylation-sensitive restriction enzyme sequencing data. [PDF]

Sci Rep, 2018
Mayne BT, Leemaqz SY, Buckberry S, Rodriguez Lopez CM, Roberts CT, Bianco-Miotto T, Breen J.   +6 more
europepmc   +1 more source

Crystal structure of DNA sequence specificity subunit of a type I restriction-modification enzyme and its functional implications

, 2005
Jeong‐Sun Kim, Andy DeGiovanni, Jaru Jancarik, Paul D. Adams, Hisao Yokota, Rosalind Kim, Sung‐Hou Kim   +6 more
openalex   +1 more source

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion. [PDF]

J Vis Exp, 2017
Santillán O, Ramírez-Romero MA, Dávila G.   +2 more
europepmc   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

In Silico Restriction Enzyme Digests to Minimize Mapping Bias in Genomic Sequencing. [PDF]

Mol Ther Methods Clin Dev, 2017
Roszik J, Fenyőfalvi G, Halász L, Karányi Z, Székvölgyi L.   +4 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source