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The Immobilization of Hyaluronic Acid in 3D Hydrogel Scaffolds Modulates Macrophage Polarization

Advanced Biology, EarlyView.
This study explores the use of collagen‐hyaluronic acid (HA) hydrogels for the 3D culture of macrophages, providing a useful tool for modelling macrophage behavior in tissues and diseases. It highlights how hydrogel composition, mechanical properties, and preparation methods influence macrophage behavior, revealing for the first time that HA's ...
Tiah CL Oates, Jasmin Boyd, Louise Dolan, C de Kergariou, Jingwen Zhu, Ash Toye, Adam W Perriman, Asme Boussahel   +7 more
wiley   +1 more source

A Twist in the Diagnosis: Chronic Arthropathy Without Inflammation

Arthritis Care &Research, EarlyView.
María Á. Puche‐Larrubia, Inmaculada C. Aranda‐Valera, Alejandro Escudero‐Contreras, Rosa Roldán‐Molina   +3 more
wiley   +1 more source

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Recognition Sequence of a Restriction Enzyme

Nature New Biology, 1973
Restriction endonuclease EcoRII makes about twenty double-stranded breaks per molecule of λh80 DNA. The 5′-terminal sequences are pC-C-A-G-G and pC-C-T-G-G. These are complementary and rotationally symmetrical, showing how the enzyme may produce DNA fragments with short cohesive ends.
Cynthia H. Bigger, Cynthia H. Bigger, Kenneth Murray, Noreen E. Murray   +3 more
openaire   +3 more sources

Restriction enzymes in cells, not eppendorfs

Trends in Microbiology, 1994
Restriction enzymes are essential reagents to molecular biologists, but their relevance to bacterial populations is less obvious. Most bacteria encode restriction and modification systems and these are commonly considered to be a barrier to phage infection. Current evidence also supports a more general role for them in genetic recombination.
Gareth King, Noreen E. Murray
openaire   +3 more sources

Restriction Enzyme Computation [PDF]

, 2003
In this paper implementation of an inner hair cell model, including macromechanics and mechanical to neural transduction process is presented and discussed. The well-known Meddis model will be use as the reference system, and a high level synthesis will provide a parametrizable implementation and a reusability code, which allows future refinements on ...
Maciej Troc, Olgierd Unold
openaire   +1 more source

Restriction & Modification Enzymes

2022
Restriction and modification enzymes serve as nature’s genetic gatekeepers. These specialized proteins function together as a defence mechanism in bacteria and protect them from invading foreign DNA, such as that of bacteriophages. Restriction enzymes, also known as restriction endonucleases, recognize specific sequences within the DNA and cleave it at
openaire   +1 more source

Engineered Calcium-Precipitable Restriction Enzyme [PDF]

ACS Synthetic Biology, 2014
We have developed a simple system for tagging and purifying proteins. Recent experiments have demonstrated that RTX (Repeat in Toxin) motifs from the adenylate cyclase toxin gene (CyaA) of B. pertussis undergo a conformational change upon binding calcium, resulting in precipitation of fused proteins and making this method a viable alternative for ...
Timothy Read, Robin D. Dowell, Josephina R. Hendrix, Jean-Francois Lalonde, Phillip K. Jensen, Michael Brasino, Sarah A. Zimmermann, William Heymann, Joseph Rokicki, Elijah Lovelace   +9 more
openaire   +2 more sources

Class-IIS restriction enzymes — a review

Gene, 1991
Class-IIS restriction enzymes (ENases-IIS) interact with two discrete sites on double-stranded DNA: the recognition site, which is 4-7 bp long, and the cleavage site, usually 1-20 bp away from the recognition site. The recognition sequences of ENases-IIS are totally (or partially) asymmetric and all of the characterized ENases-IIS are monomeric.
Szybalski, W, Kim, SC Kim, Sun-Chang, Hasan, N, Podhajska, AJ   +3 more
openaire   +4 more sources

ATP-dependent restriction enzymes

2000
The phenomenon of restriction and modification (R-M) was first observed in the course of studies on bacteriophages in the early 1950s. It was only in the 1960s that work of Arber and colleagues provided a molecular explanation for the host specificity.
Rao, Desirazu N, Saha, Swati, Krishnamurthy, Vinita   +2 more
openaire   +3 more sources

Restriction Enzymes and DNA

1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
openaire   +2 more sources