Expression and purification of the modification-dependent restriction enzyme BisI and its homologous enzymes. [PDF]
Xu SY+3 more
europepmc +1 more source
Time‐Controlled Dual Targeting to Program Systemic and Intercellular Transfer of Therapeutic Effects
Aspirin‐liposomes loaded onto monocytes enable inflammation‐triggered targeting and efficient hand‐over of aspirin to inflamed cells. Monocytes uptake a significant portion of aspirin‐liposomes, prolonging therapeutic action. This approach enhances anti‐inflammatory effects through intercellular transfer, demonstrating a translational strategy for ...
Seung Eun Yu+6 more
wiley +1 more source
Characterization of eukaryotic DNA N(6)-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. [PDF]
Luo GZ+8 more
europepmc +1 more source
Mesenchymal stem cell‐derived nanoghosts (MSC‐NGs) mimic naturally secreted extracellular vesicles (MSC‐EVs) in structure and physicochemical properties but can be synthesized at more translatable yields. As osteogenic agents, MSC‐NGs demonstrate superior outcomes compared to MSC‐EVs.
Antoine Karoichan+4 more
wiley +1 more source
Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme. [PDF]
Csefalvay E+13 more
europepmc +1 more source
Sonochemical Functionalization of Glass
A new method for functionalizing glass using ultrasonication of aryl diazonium salts. Aryl radicals generated by ultrasounds react with glass, forming a stable, covalently bonded organic film. The functionalized glass adheres microorganisms such as microalgae, fungi, and bacteria, suggesting potential applications in enzyme production, filtration ...
Tiexin Li+13 more
wiley +1 more source
Inquiry-based experiments for large-scale introduction to PCR and restriction enzyme digests. [PDF]
Johanson KE, Watt TJ.
europepmc +1 more source
Electric Pulse Regulated MXene Based Nanozymes for Integrative Bioelectricity Immuno‐Cancer Therapy
MXenzyme‐mediated bioelectricity cancer therapy (MXenzyme‐BECT) enhances cancer cell death through irreversible depolarization, ion channel disruption, ROS generation, and immunogenic cell death. Computational simulations reveal the electrical mechanisms by which MXenzyme acts on single cells and support to predict treatment parameters. Next‐generation
Sanghee Lee+6 more
wiley +1 more source
Genomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP. [PDF]
Mandakovic D+6 more
europepmc +1 more source