Results 301 to 310 of about 2,927,400 (332)
Some of the next articles are maybe not open access.
, 1995
To use insertional mutagenesis for gene tagging, we tested whether transformation of Magnaporthe grisea could be enhanced by restriction enzyme mediated integration (REMI) of plasmid DNA.
Z. Shi, D. Christian, H. Leung
semanticscholar +1 more source
To use insertional mutagenesis for gene tagging, we tested whether transformation of Magnaporthe grisea could be enhanced by restriction enzyme mediated integration (REMI) of plasmid DNA.
Z. Shi, D. Christian, H. Leung
semanticscholar +1 more source
[21] Restriction enzymes: Properties and use
1992Publisher Summary This chapter lists all commercially available enzymes, to describe their general properties, the basic procedures for their use, and ways of troubleshooting problems encountered in their use. Although the number of enzymes being discussed is large, a few simple rules apply to virtually all the enzymes.
openaire +3 more sources
Understanding Restriction Enzyme Digests
2021Prior to submitting genomic DNA for sequencing, SEA students perform a restriction endonuclease digest with several enzymes, which provides a fast and cost-effective way of quickly screening isolated phages. The pattern of bands on the gel should be unique except for very closely related or identical phages, which is useful when trying to identify ...
Dustin Edwards +4 more
openaire +2 more sources
Caloric restriction, aging, and antioxidant enzymes
Mutation Research/DNAging, 1993The basic mechanisms of aging and its retardation by caloric restriction (CR) remain unclear. One suggested means by which CR could retard aging is based on production of mitochondrial free radicals, and efficiency of their subsequent metabolism. Currently, there is little information concerning the influences of age and CR on the rates of in vivo ...
Ronald W. Hart +2 more
openaire +3 more sources
Journal of Medical and Veterinary Mycology, 1993
We have developed a method for processing and detecting fungi in clinical specimens using the polymerase chain reaction (PCR) methodology. This PCR amplification of a segment of a ribosomal DNA gene results in a 310 bp product. The gene sequences used as
R. Hopfer +3 more
semanticscholar +1 more source
We have developed a method for processing and detecting fungi in clinical specimens using the polymerase chain reaction (PCR) methodology. This PCR amplification of a segment of a ribosomal DNA gene results in a 310 bp product. The gene sequences used as
R. Hopfer +3 more
semanticscholar +1 more source
Restriction Enzyme Analysis of PCR Products
2009Progress in single nucleotide polymorphism (SNP) detection technologies has provided information for SNP-based studies, such as identification of candidate genes for the complex genetic diseases, pharmacogenetic analysis, drug development, population genetics, evolutionary studies, and forensic investigations. SNP detection is performed by many methods,
Hideki Asamura +3 more
openaire +3 more sources
Restriction Enzymes from Thermophiles
2013Restriction endonucleases (REases) are enzymes that recognize and cleave DNA in a sequence specific manner. The recognition site consists of a sequence of nucleotides in the DNA duplex, typically four to eight base pairs long. Most of the commercially produced REases are isolated from the mesophilic bacteria.
Prince Sharma +2 more
openaire +2 more sources
High Throughput Cloning with Restriction Enzymes
2008The systematic structural analysis of many target proteins involves generating expression clones in high throughput. This requires robust laboratory procedures and benefits from laboratory automation and data management systems. This chapter gives an overview of the Protein Structure Factory, a structural genomics project focusing on human proteins ...
Asgar Ergin +2 more
openaire +3 more sources
PNAzymes That Are Artificial RNA Restriction Enzymes
Journal of the American Chemical Society, 2010DNA-cleaving restriction enzymes are well-known tools in biomedical and biotechnological research. There are, however, no corresponding enzymes known for RNA cleavage. There has been an ongoing development of artificial ribonucleases, including some attempts at sequence selectivity. However, so far these systems have displayed modest rates of cleavage,
Merita Murtola +2 more
openaire +3 more sources
DNAase I footprinting of restriction enzymes
Biochemical and Biophysical Research Communications, 1988DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs.
openaire +3 more sources