Results 71 to 80 of about 3,197,803 (398)
Alleviation of restriction by DNA condensation and non-specific DNA binding ligands [PDF]
, 2004 During conditions of cell stress, the type I restriction and modification enzymes of bacteria show reduced, but not zero, levels of restriction of unmethylated foreign DNA.
Dryden, D T F, Keatch, S A, Su, T J
core +2 more sources
Accelerated search kinetics mediated by redox reactions of DNA repair enzymes [PDF]
Biophysical Journal, volume 96, pp 3949-3958, (2009), 2009 A Charge Transport (CT) mechanism has been proposed in several papers (e.g., Yavin, et al. PNAS, v102 3546 (2005)) to explain the localization of Base Excision Repair (BER) enzymes to lesions on DNA. The CT mechanism relies on redox reactions of iron-sulfur cofactors that modify the enzyme's binding affinity.
arxiv +1 more source
Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar +2 more
openaire +3 more sources
Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia
FEBS Letters, EarlyView.This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley +1 more source
Research Journal of Life Science, 2015 Research with aim to estimate genetic polymorphism at ACACA (Acetyl-coenzyme A carboxylase ) locus in Ettawa Crossbred goat wan its relationship with production traits was done at goat population in Batu, Lawang and Ampel Gading.
Sucik Maylinda +2 more
doaj +1 more source
RFLP of 16S-ITS rDNA region to differentiate Lactobacilli at species level [PDF]
, 2004 The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI ...
Bulut, Çisem +4 more
core +1 more source
Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences [PDF]
, 2019 Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence. Using information theory we develop an equation that defines the number of independent contacts, which is the dimensionality of the binding. We show that EcoRI, which
arxiv +1 more source
Chimeric Restriction Enzymes: What Is Next? [PDF]
Biological Chemistry, 1999 Chimeric restriction enzymes are a novel class of engineered nucleases in which the non-specific DNA cleavage domain of Fokl (a type IIS restriction endonuclease) is fused to other DNA-binding motifs. The latter include the three common eukaryotic DNA-binding motifs, namely the helix-turn-helix motif, the zinc finger motif and the basic helix-loop ...
Srinivasan Chandrasegaran, Jeff Smith
openaire +3 more sources
Diphthamide synthesis is linked to the eEF2‐client chaperone machinery
FEBS Letters, EarlyView.The diphthamide modification of eukaryotic translation elongation factor 2 (eEF2) is important for accurate protein synthesis. We addressed the potential coordination of de novo eEF2 synthesis with simultaneous or subsequent diphthamide modification. Our work reveals that the co‐chaperones Hgh1 and Cpr7, which are known to support folding of nascent ...
Lars Kaduhr +4 more
wiley +1 more source
Exploration of MedicineStreptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on ...
Marina Yusoff +3 more
doaj +1 more source