Results 81 to 90 of about 347,445 (313)

A general method for the purification of restriction enzymes

Nucleic Acids Research, 1978
An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
Francisco Bolívar, Raymond L. Rodriguez, David J. Russel, Robert C. Tait, Herbert W. Boyer, Alejandra A. Covarrubias, Herbert L. Heyneker, Mary C. Betlach, Patricia J. Greene, Keith Backman   +9 more
openaire   +4 more sources

Isolation of large molecular weight DNA from agarose gels forfurther digestion by restriction enzymes [PDF]

, 1975
Nikolaus Blin, Alexander von Gabain, Hermann Bujard   +2 more
openalex   +1 more source

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

FEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman, Annika Egeler, Saskia Rödl, Johannes M. Herrmann   +3 more
wiley   +1 more source

SNP-RFLPing 2: an updated and integrated PCR-RFLP tool for SNP genotyping

BMC Bioinformatics, 2010
Background PCR-restriction fragment length polymorphism (RFLP) assay is a cost-effective method for SNP genotyping and mutation detection, but the manual mining for restriction enzyme sites is challenging and cumbersome.
Chang Hsueh-Wei, Cheng Yu-Huei, Chuang Li-Yeh, Yang Cheng-Hong   +3 more
doaj   +1 more source

Restoration by T4 ligase of DNA sequences sensitive to “flush”-cleaving restriction enzyme [PDF]

, 1977
Monica Mottes, Carlo Morandi, S. Cremaschi, V. Sgaramella   +3 more
openalex   +1 more source

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source

Restriction enzyme cleavage sites surrounding the structural gene for the lipoprotein of the Escherichia coli outer membrane [PDF]

, 1979
K. Nakamura, Eleanore T. Wurtzel, Robert M. Pirtle, Masayori Inouye   +3 more
openalex   +1 more source

Tonic signaling of the B‐cell antigen‐specific receptor is a common functional hallmark in chronic lymphocytic leukemia cell phosphoproteomes at early disease stages

Molecular Oncology, EarlyView.
B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez, Pablo Juanes‐Velasco, Marina L. García‐Vaquero, Conrad Droste, Alicia Landeira‐Viñuela, Miguel Alcoceba, Helena Fidalgo‐Gómez, Sara Misiego‐Herrero, Almudena Navarro‐Bailón, Mónica Baile, José M. Bastida, Jose Manuel Sanchez‐Santos, Rafael Góngora, Julia Almeida, Marcos Gonzalez‐Diaz, Alberto Orfao, Javier De Las Rivas, Manuel Fuentes   +17 more
wiley   +1 more source

Structural and functional analysis of the symmetrical Type I restriction endonuclease R.EcoR124I_NT

PLoS ONE, 2012
Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E Taylor, Anna Swiderska, Jean-Baptiste Artero, Philip Callow, Geoff Kneale   +4 more
doaj   +1 more source

Sites of cleavage by restriction enzymes in viral DNAs: comparison of observed and expected frequencies [PDF]

, 1974
R. A. Elton
openalex   +1 more source