Results 81 to 90 of about 3,197,803 (398)

PCR-RFLP Using BseDI Enzyme for Pork Authentication in Sausage and Nugget Products [PDF]

, 2011
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying ...
Abidin, M. Z. (M), Erwanto, Y. (Y), Rohman, A. (A), Sismindari, S. (Sismindari)   +3 more
core  

Enzyme oscillation can enhance the thermodynamic efficiency of cellular metabolism: Consequence of anti-phase coupling between reaction flux and affinity [PDF]

, 2015
Cells generally convert nutrient resources to useful products via energy transduction. Accordingly, the thermodynamic efficiency of this conversion process is one of the most essential characteristics of living organisms. However, although these processes occur under conditions of dynamic metabolism, most studies of cellular thermodynamic efficiency ...
arxiv   +1 more source

Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β‐carbonic anhydrase

FEBS Letters, EarlyView.
Here, we performed a functional analysis of the fungal enzymes belonging to clade D of the β‐class carbonic anhydrase family (β‐D‐CA). The β‐D‐CAs in the basidiomycete Gloeophyllum trabeum and the ascomycete Trichoderma harzianum showed very low activity in the hydration of CO2 but exhibited high activity in the hydrolysis of carbonyl sulfide (COS ...
Ryuka Iizuka, Takahiro Ogawa, Rikako Tsukida, Keiichi Noguchi, David Hibbett, Yoko Katayama, Makoto Yoshida   +6 more
wiley   +1 more source

DNA Mapping Algorithms: Strategies for Single Restriction Enzyme and Multiple Restriction Enzyme Mapping [PDF]

, 1992
An approach to high-resolution restriction-fragment DNA mapping, known as Multiple-Restriction-Enzyme mapping (MRE mapping), is present. This approach significantly reduces the uncertainty of clone placement by using clone ends to synchronize the ...
Gillett, Will
core   +1 more source

Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.

Proceedings of the National Academy of Sciences of the United States of America, 1992
An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events. Restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2)
G. Walker, M. C. Little, J. G. Nadeau, D. D. Shank   +3 more
semanticscholar   +1 more source

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

FEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley, Daniel H. Shain, Eric A. Klein   +2 more
wiley   +1 more source

Type II restriction modification system in Ureaplasma parvum OMC-P162 strain. [PDF]

PLoS ONE, 2018
Ureaplasma parvum serovar 3 strain, OMC-P162, was isolated from the human placenta of a preterm delivery at 26 weeks' gestation. In this study, we sequenced the complete genome of OMC-P162 and compared it with other serovar 3 strains isolated from ...
Heng Ning Wu, Yukiko Nakura, Michinobu Yoshimura, Ourlad Alzeus Gaddi Tantengco, Makoto Nomiyama, Toshimitsu Takayanagi, Tomio Fujita, Kiyoshi Yasukawa, Itaru Yanagihara   +8 more
doaj   +1 more source

Further evidence to justify reassignment of Mycoplasma mycoides subspecies mycoides Large Colony type to Mycoplasma mycoides subspecies capri [PDF]

, 2010
Analysis, using the polymerase chain reaction (PCR), restriction enzyme endonuclease analysis (REA), protein profile patterns, random amplification of polymorphic DNA (RAPD) fingerprinting, 16S rRNA gene sequencing and antisera growth inhibition tests ...
Abu-Groun, Al-Aubaidi, Ann P. Wood, Artiushin, Bashiruddin, Bashiruddin, Borrel, Bové, Bradbury, Brown, Butler, Christiansen, Costas, Cottew, Cottew, Cottew, Donovan P. Kelly, Edward, Fan, Fitzmaurice, Hall, Harasawa, Johansson, Johansson, Laemmli, Leach, Leach, Lin, Manso-Silvan, Manso-Silvan, Masoud Shahram, Monnerat, Nowak, Pettersson, Poveda, Rawadi, Rawadi, Robin A.J. Nicholas, Rodwell, Rodwell, Stakenborg, Tardy, Taylor, Taylor, Thiaucourt, Thigpen, Ursi, Vilei, Wang   +48 more
core   +1 more source

Fast-scan atomic force microscopy reveals that the type III restriction enzyme EcoP15I is capable of DNA translocation and looping

Proceedings of the National Academy of Sciences of the United States of America, 2007
Many DNA-modifying enzymes act in a manner that requires communication between two noncontiguous DNA sites. These sites can be brought into contact either by a diffusion-mediated chance interaction between enzymes bound at the two sites, or by active ...
N. Crampton, M. Yokokawa, D. Dryden, J. Edwardson, D. Rao, K. Takeyasu, S. Yoshimura, R. Henderson   +7 more
semanticscholar   +1 more source

Functional variation among LPMOs revealed by the inhibitory effects of cyanide and buffer ions

FEBS Letters, EarlyView.
This study addresses the inhibition of lytic polysaccharide monooxygenases (LPMOs) by cyanide and explains how and why the magnitude of observed inhibitory effects depends on the way LPMO reactions are setup and on the type of LPMO. Enzymes known as lytic polysaccharide monooxygenases (LPMOs) are mono‐copper polysaccharide‐degrading peroxygenases that ...
Ole Golten, Lorenz Schwaiger, Zarah Forsberg, Kelsi R. Hall, Anton A. Stepnov, Tom Z. Emrich‐Mills, Iván Ayuso‐Fernández, Morten Sørlie, Roland Ludwig, Åsmund Kjendseth Røhr, Vincent G. H. Eijsink   +10 more
wiley   +1 more source