Results 1 to 10 of about 280,842 (293)

Telomere Restriction Fragment (TRF) Analysis [PDF]

open access: yesBio-Protocol, 2015
While telomerase is expressed in ~90% of primary human tumors, most somatic tissue cells except transiently proliferating stem-like cells do not have detectable telomerase activity (Shay and Wright, 1996; Shay and Wright, 2001).
ilgen Mender, Jerry Shay
doaj   +6 more sources

HIFI: estimating DNA-DNA interaction frequency from Hi-C data at restriction-fragment resolution [PDF]

open access: yesGenome Biology, 2020
Hi-C is a popular technique to map three-dimensional chromosome conformation. In principle, Hi-C’s resolution is only limited by the size of restriction fragments.
Christopher JF Cameron   +2 more
doaj   +2 more sources

Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism [PDF]

open access: yesDiagnostics, 2019
Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera.
María del Mar García-Suárez   +5 more
doaj   +2 more sources

Secure and optimized detection of PNPLA3 rs738409 genotype by an improved PCR–restriction fragment length polymorphism method

open access: yesBioTechniques, 2021
The PNPLA3 reference single-nucleotide polymorphism rs738409 has been identified as a predisposing factor for nonalcoholic fatty liver disease. A simple method based on PCR and restriction fragment length polymorphism (RFLP) analysis had been published ...
Jörg Enssle, Karsten-Henrich Weylandt
doaj   +1 more source

RESTseq--efficient benchtop population genomics with RESTriction Fragment SEQuencing. [PDF]

open access: yesPLoS ONE, 2013
We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers.
Eckart Stolle, Robin F A Moritz
doaj   +1 more source

Simplified DGS procedure for large-scale genome structural study

open access: yesBioTechniques, 2009
Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure.
Yong-Chul Jung   +5 more
doaj   +1 more source

Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene

open access: yesMemorias do Instituto Oswaldo Cruz, 2014
An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses.
Vítor Yamashiro Rocha Soares   +9 more
doaj   +1 more source

RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains

open access: yesMemorias do Instituto Oswaldo Cruz, 2009
Restriction fragment length polymorphism (RFLP) analysis of a PCR-amplified fragment of the 16S rRNA gene was performed on reference strains belonging to 21 different enterococcal species and on 75 Enterococcus isolates recovered from poultry meat ...
EMD Scheidegger   +3 more
doaj   +1 more source

Differentiation of Meat Samples from Domestic Horses ( Equus caballus ) and Asiatic Wild Asses ( Equus hemionus ) Using a Species-Speci fi c Restriction Site in the Mitochondrial Cytochrome b Region [PDF]

open access: yesMongolian Journal of Biological Sciences, 2006
Recent studies suggest that Asiatic wild asses ( Equus hemionus ) are being increasingly poached in a commercial fashion. Part of the meat is believed to reach the meat markets in the capital Ulaanbaatar.
Ralph Kuehn   +4 more
doaj   +1 more source

Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP

open access: yesJournal of Biological Researches, 2012
This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro   +3 more
doaj   +1 more source

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