Results 1 to 10 of about 562,575 (339)

Telomere Restriction Fragment (TRF) Analysis [PDF]

open access: yesBio-Protocol, 2015
While telomerase is expressed in ~90% of primary human tumors, most somatic tissue cells except transiently proliferating stem-like cells do not have detectable telomerase activity (Shay and Wright, 1996; Shay and Wright, 2001).
ilgen Mender, Jerry Shay
doaj   +5 more sources

HIFI: estimating DNA-DNA interaction frequency from Hi-C data at restriction-fragment resolution [PDF]

open access: yesGenome Biology, 2020
Hi-C is a popular technique to map three-dimensional chromosome conformation. In principle, Hi-C’s resolution is only limited by the size of restriction fragments.
Christopher JF Cameron   +2 more
doaj   +2 more sources

Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism [PDF]

open access: yesDiagnostics, 2019
Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera.
María del Mar García-Suárez   +5 more
doaj   +2 more sources

Secure and optimized detection of PNPLA3 rs738409 genotype by an improved PCR–restriction fragment length polymorphism method

open access: yesBioTechniques, 2021
The PNPLA3 reference single-nucleotide polymorphism rs738409 has been identified as a predisposing factor for nonalcoholic fatty liver disease. A simple method based on PCR and restriction fragment length polymorphism (RFLP) analysis had been published ...
Jörg Enssle, Karsten-Henrich Weylandt
doaj   +1 more source

RESTseq--efficient benchtop population genomics with RESTriction Fragment SEQuencing. [PDF]

open access: yesPLoS ONE, 2013
We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers.
Eckart Stolle, Robin F A Moritz
doaj   +1 more source

Restriction Fragment Analysis of Pine Phylogeny [PDF]

open access: yesEvolution, 1990
We used restriction fragment analysis of chloroplast, nuclear, and mitochondrial DNA to study phylogeny in the genus Pinus. Total genomic DNA of 18 to 19 pine species that spanned 14 of the 15 subsections in the genus was cut with 8 restriction enzymes, blotted, and then probed with up to 17 cloned DNA fragments-which were mostly from the chloroplast ...
Steven H, Strauss, Allan H, Doerksen
openaire   +2 more sources

Simplified DGS procedure for large-scale genome structural study

open access: yesBioTechniques, 2009
Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure.
Yong-Chul Jung   +5 more
doaj   +1 more source

Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene

open access: yesMemorias do Instituto Oswaldo Cruz, 2014
An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses.
Vítor Yamashiro Rocha Soares   +9 more
doaj   +1 more source

RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains

open access: yesMemorias do Instituto Oswaldo Cruz, 2009
Restriction fragment length polymorphism (RFLP) analysis of a PCR-amplified fragment of the 16S rRNA gene was performed on reference strains belonging to 21 different enterococcal species and on 75 Enterococcus isolates recovered from poultry meat ...
EMD Scheidegger   +3 more
doaj   +1 more source

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