Results 151 to 160 of about 276,996 (189)

Small-fragment restriction endonuclease analysis in epidemiological mapping of group A streptococci

open access: bronze, 1994
Haima Mylvaganam   +5 more
openalex   +1 more source

Chromatographic separation of DNA restriction fragments

Journal of Chromatography B: Biomedical Sciences and Applications, 1987
Reversed-phase liquid chromatography (RPC-5) was the first chromatographic technique to be successfully applied to DNA restriction fragments. Size-exclusion high-performance liquid chromatography (HPLC) has the advantage of wide variability in buffer conditions but is restricted to an upper size limit at 800 base pairs.
Rolf Hecker, Detlev Riesner
openaire   +3 more sources

Optimization of Restriction Fragment DNA Mapping

Journal of Computational Biology, 1998
Consider a mapping project in which overlap of clonal segments is inferred from complete multiple restriction digests. The fragment sizes of the clones are measured with some error, potentially leading to a map with erroneous links. The number of errors in the map depends on the number and types of enzymes used to characterize the clones.
Jared C. Roach   +4 more
openaire   +3 more sources

Immunoglobulin genes in DNA restriction fragments

Biochemistry, 1978
We have investigated the organization of immunoglobulin genes in mice. High molecular weight DNA from myelomas and Krebs ascites cells was cleaved with EcoRI restriction endonuclease and fractionated using preparative agarose gel electrophoresis. Each fraction was then hybridized to an immunoglobulin mRNA or a cDNA transcribed from the mRNA.
Alan M. Walfield   +3 more
openaire   +2 more sources

Restriction fragment length polymorphism [PDF]

open access: possible, 1988
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA.
openaire   +1 more source

Mapping the order of DNA restriction fragments

Gene, 1983
A straightforward method was designed for mapping the order of DNA restriction fragments obtained by a double and two single digestions, without the necessity of using a computer or a radioactive label. All possible solutions compatible with a pre-set level of error in the determination of sequence lengths are obtained.
William W. Ralph   +2 more
openaire   +3 more sources

Restriction Fragment Length Polymorphism Analysis

Current Protocols in Human Genetics, 1994
AbstractThis unit describes Restriction Fragment Length Polymorphism (RFLP) analysis, which utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA regions of interest. When investigating families for inheritance of an RFLP, aliquots of genomic DNA from individual family members are digested to completion with
openaire   +3 more sources

Antibody of Restricted Heterogeneity secreted by Spleen Fragments [PDF]

open access: possibleNature New Biology, 1972
SMALL fragments of lymphoid tissue in culture will incorporate radioactive amino-acids into secreted immunoglobulin. Electrophoresis followed by autoradiography has been used to indicate the degree of heterogeneity of the immunoglobulin1–5. Fragments of lymph node from a rabbit immunized with low doses of Salmonella organisms2–4 and of spleens from ...
Anne Cooke, Arnold Feinstein, John North
openaire   +2 more sources

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