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Small-fragment restriction endonuclease analysis in epidemiological mapping of group A streptococci
Haima Mylvaganam+5 more
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Chromatographic separation of DNA restriction fragments
Journal of Chromatography B: Biomedical Sciences and Applications, 1987Reversed-phase liquid chromatography (RPC-5) was the first chromatographic technique to be successfully applied to DNA restriction fragments. Size-exclusion high-performance liquid chromatography (HPLC) has the advantage of wide variability in buffer conditions but is restricted to an upper size limit at 800 base pairs.
Rolf Hecker, Detlev Riesner
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Optimization of Restriction Fragment DNA Mapping
Journal of Computational Biology, 1998Consider a mapping project in which overlap of clonal segments is inferred from complete multiple restriction digests. The fragment sizes of the clones are measured with some error, potentially leading to a map with erroneous links. The number of errors in the map depends on the number and types of enzymes used to characterize the clones.
Jared C. Roach+4 more
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Immunoglobulin genes in DNA restriction fragments
Biochemistry, 1978We have investigated the organization of immunoglobulin genes in mice. High molecular weight DNA from myelomas and Krebs ascites cells was cleaved with EcoRI restriction endonuclease and fractionated using preparative agarose gel electrophoresis. Each fraction was then hybridized to an immunoglobulin mRNA or a cDNA transcribed from the mRNA.
Alan M. Walfield+3 more
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Restriction fragment length polymorphism [PDF]
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA.
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Mapping the order of DNA restriction fragments
Gene, 1983A straightforward method was designed for mapping the order of DNA restriction fragments obtained by a double and two single digestions, without the necessity of using a computer or a radioactive label. All possible solutions compatible with a pre-set level of error in the determination of sequence lengths are obtained.
William W. Ralph+2 more
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Restriction Fragment Length Polymorphism Analysis
Current Protocols in Human Genetics, 1994AbstractThis unit describes Restriction Fragment Length Polymorphism (RFLP) analysis, which utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA regions of interest. When investigating families for inheritance of an RFLP, aliquots of genomic DNA from individual family members are digested to completion with
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Antibody of Restricted Heterogeneity secreted by Spleen Fragments [PDF]
SMALL fragments of lymphoid tissue in culture will incorporate radioactive amino-acids into secreted immunoglobulin. Electrophoresis followed by autoradiography has been used to indicate the degree of heterogeneity of the immunoglobulin1–5. Fragments of lymph node from a rabbit immunized with low doses of Salmonella organisms2–4 and of spleens from ...
Anne Cooke, Arnold Feinstein, John North
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