Results 11 to 20 of about 280,842 (293)
Restriction fragment length polymorphism (RFLP) [PDF]
American Journal of Physical Anthropology, 1989 A restriction fragment length polymorphism (RFLP) is defined by an enzyme, a restriction endonuclease, that cuts the doublestranded DNA at a particular sequence of bases, a probe, a labeled, complementary segment of DNA that will anneal to a portion of the digested sample, and a set of variable fragment length bands that appear on a Southern blot.
Robert C. Williams
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A method for sequencing restriction fragments with dideoxynucleoside triphosphates [PDF]
Nucleic Acids Research, 1978 A rapid enzymatic approach is described for the sequence analysis of a 5' terminally labelled restriction fragment. It involves limited nicking of the strands of the molecule throughout the sequence by pancreatic DNAase I. The 3' hydroxyl groups exposed by each nick are then used to prime chain extension by DNA polymerase I in four separate reactions ...
J. Maat, Andrew J.H. Smith
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Restriction Fragment Analysis of Pine Phylogeny [PDF]
Evolution, 1990 We used restriction fragment analysis of chloroplast, nuclear, and mitochondrial DNA to study phylogeny in the genus Pinus. Total genomic DNA of 18 to 19 pine species that spanned 14 of the 15 subsections in the genus was cut with 8 restriction enzymes, blotted, and then probed with up to 17 cloned DNA fragments-which were mostly from the chloroplast ...
Steven H. Strauss, Allan H. Doerksen
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European Journal of Entomology, 2008 Psocids of the genus Liposcelis (Psocoptera: Liposcelididae) are stored product pests that are difficult to identify morphologically. A molecular method based on Restriction Fragment Length Polymorphism (RFLP) of the PCR-amplified 16S rDNA gene was ...
Meng QIN +4 more
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RestrictionDigest: A powerful Perl module for simulating genomic restriction digests
Electronic Journal of Biotechnology, 2016 Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang +4 more
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Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort [PDF]
International Journal of Fertility and Sterility, 2017 Background: Infertility is the inability of a couple to conceive after one and a half years of unprotected sex. Male infertility, which accounts for almost half of infertility cases, is considered as a major problem all over the world.
Shalaka S Ramgir +3 more
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Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi
The Plant Pathology Journal, 2016 Gene disruption by homologous recombination is widely used to investigate and analyze the function of genes in Fusarium fujikuroi, a fungus that causes bakanae disease and root rot symptoms in rice.
In Sun Hwang, Il-Pyung Ahn
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Anais Brasileiros de Dermatologia, 2010 Os autores descrevem caso de pitiríase versicolor circinada, cujo agente etiológico isolado foi Malassezia sympodialis em uma mulher de 34 anos. O isolamento e identificação da Malassezia sympodialis foi em ágar Dixon modificado e o método molecular para
Valéria Maria de Souza Framil +4 more
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Identification of beef using restriction fragment length polymorphism– [PDF]
Iraqi Journal of Veterinary Sciences, 2009 To differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to
R. A. Al-Sanjary
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A strategy for seamless cloning of large DNA fragments from Streptomyces
BioTechniques, 2015 We report a novel method for the seamless cloning of large DNA fragments (SCLF) of up to 44 kb or larger from Streptomyces chromosomal DNA. SCLF is based on homologous recombination in Streptomyces and is easy to perform. The strategy of SCLF is to flank
Jun Huang +5 more
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