Results 261 to 270 of about 280,842 (293)

Mapping the order of DNA restriction fragments

Gene, 1983
A straightforward method was designed for mapping the order of DNA restriction fragments obtained by a double and two single digestions, without the necessity of using a computer or a radioactive label. All possible solutions compatible with a pre-set level of error in the determination of sequence lengths are obtained.
William W. Ralph, Walter M. Fitch, Temple F. Smith   +2 more
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Restriction fragment length polymorphism [PDF]

, 1988
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA.
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Restriction Fragment Length Polymorphism Analysis

Current Protocols in Human Genetics, 1994
AbstractThis unit describes Restriction Fragment Length Polymorphism (RFLP) analysis, which utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA regions of interest. When investigating families for inheritance of an RFLP, aliquots of genomic DNA from individual family members are digested to completion with
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Antibody of Restricted Heterogeneity secreted by Spleen Fragments [PDF]

Nature New Biology, 1972
SMALL fragments of lymphoid tissue in culture will incorporate radioactive amino-acids into secreted immunoglobulin. Electrophoresis followed by autoradiography has been used to indicate the degree of heterogeneity of the immunoglobulin1–5. Fragments of lymph node from a rabbit immunized with low doses of Salmonella organisms2–4 and of spleens from ...
Anne Cooke, Arnold Feinstein, John North
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[9] Gel electrophoresis of restriction fragments

1979
Publisher Summary This chapter focuses on gel electrophoresis of restriction fragments. There are many designs of apparatus that can be used for the electrophoresis of DNA in agarose or polyacrylamide gels. EtBr may be included in the gel and running buffers; it has only a small effect on the mobility of DNA.
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Restriction fragment length polymorphisms of Anisakinae larvae

Journal of Helminthology, 1989
ABSTRACTThe analysis of restriction fragment length polymorphisms (RFLPs) was applied to distinguish several kinds of Anisakinae larvae, Anisakis larvae (type I) collected from two different paratenic hosts, Anisakis larvae (type II) and Contracaecum larvae. The patterns of the two different paratenic host-derived DNA of Anisakis larva (I) were exactly
Tadashi Matsuura, Kazuo Sugane, Liu Qing
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Restriction Fragment Length Polymorphism Typing of Mycobacteria

2003
In principle, restriction fragment length polymorphism (RFLP) typing can be applied to strains of all mycobacterial species for which suitable probes have been identified. International consensus has been achieved regarding the methodology of IS6110 RFLP typing of Mycobacterium tuberculosis complex isolates (1) and IS1245 RFLP typing of Mycobacterium ...
van Soolingen, Drivm, de Haas, PEW, Kremer, Krivm   +2 more
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Economic and Fragmentation Effects of Clearcut Restrictions

Forest Science, 1998
Abstract Clearcut restrictions limiting individual clearcut size affect economic outputs and alter the spatial distribution of wildlife habitat over the landscape. Simulations of different clearcutting restrictions were applied to a 47,500 ha mixed-ownership landscape in the Sierra Nevada of California.
Tara M. Barrett, L. S. Davis, J. K. Gilless   +2 more
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Telomeric Terminal Restriction Fragment (TRF)

2003
Telomeres are protein-DNA structures at the ends of all eukaryotic chromosomes that are maintained by a unique ribonucleoprotein known as telomerase. This highly specialized RNA-dependent DNA polymerase provides a critical solution to the end-replication problem by adding TTAGGG repeats to 3' ends of human chromosomes (1-3). Telomere regulation is both
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Integrated Microdevice for DNA Restriction Fragment Analysis

Analytical Chemistry, 1996
An integrated monolithic device (8 mm × 10 mm) that performs an automated biochemical procedure is demonstrated. The device mixes a DNA sample with a restriction enzyme in a 0.7-nL reaction chamber and after a digestion period injects the fragments onto a 67-mm-long capillary electrophoresis channel for sizing. Materials are precisely manipulated under
John Michael Ramsey, Stephen C. Jacobson
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