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Multiplex-terminal restriction fragment length polymorphism
Nature Protocols, 2006A novel method called "multiplex-terminal restriction fragment length polymorphism (M-TRFLP)" has been recently developed which can be used for simultaneous analysis of the community composition of two or more microbial taxa (up to four). This method can also be used for microbial diagnostic purposes. For M-TRFLP analysis, primers specific to different
Singh, Brajesh K. (R15253) +1 more
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Restriction fragment analysis of somaclones
1991Somaclonal variation is a feature common to all plant regeneration via a tissue culture or ‘callus’ phase [7, 10]. Whether the intention is to maximise variation (for production of variability in plant breeding) or minimise it (to reduce variability in mass propagation systems or transformation studies) it is very important to be able to identify ...
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Variability for restriction fragment lengths and phylogenies in lentil
Theoretical And Applied Genetics, 1989Thirty accessions of domesticated (Lens culinaris ssp. culinaris) and wild (L. culinaris ssp. orientalis, L. culinaris ssp. odemensis, L. nigricans ssp. ervoides and L. nigricans ssp. nigricans) lentil were evaluated for restriction fragment length polymorphisms (RFLPs) using ten relative low-copy-number probes selected from partial genomic and cDNA ...
Fred J. Muehlbauer, Michael J. Havey
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Restriction fragment analysis of the secalin loci of rye
Biochemical Genetics, 1992Analyses of wheat/rye addition lines by Southern blotting confirmed the presence of sequences related to the Sec 1, Sec 2, and Sec 3 loci on chromosomes 1R and 2R. Comparison of the 1R and 2R addition lines allowed the identification of gamma-secalin genes at Sec 1 and Sec 2, respectively, while omega-secalin and gamma-secalin genes at Sec 1 were ...
Paolo A. Sabelli +2 more
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Renin locus restriction fragment length polymorphism
Human Genetics, 1989The use of two genomic EcoRI fragments as probes is discussed.
Liang Zhong Chen, Simon Easteal
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Restriction fragment length polymorphism diversity in soybean
Theoretical And Applied Genetics, 1989Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers
Reid G. Palmer +2 more
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Anion-exchange chromatography of DNA restriction fragments
Journal of Chromatography A, 1991The abilities of several high-performance liquid chromatography (HPLC) anion-exchange packings to separate DNA restriction fragments, ranging in size from 50 to 23,000 base pairs, were studied. The ion exchangers investigated include the porous packings Protein-Pak DEAE-5PW, Nucleogen-DEAE 4000-7, Poros-Q and BakerBond WP-PEI, and the non-porous ...
Marka A. Strege, Avinash L. Lagu
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Preferential nucleosome placement on pBR322 restriction fragments
Biochemical and Biophysical Research Communications, 1986Two restriction fragments of DNA containing the regulatory feature GTG/CAC were experimentally associated with core histones. The reconstituted DNA-histone complexes consisted of different forms of mononucleosomes. Lambda exonuclease and Fnu4HI were used to probe the structure of each distinct nucleoprotein complex.
I. Winicov +2 more
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Chromosome 13 restriction fragment length polymorphisms
Human Genetics, 1984The gene locus for hereditary retinoblastoma is on human chromosome 13, band q14. With this gene localization in mind, we cloned DNA fragments from this chromosome. Three of the fragments identify restriction fragment length polymorphisms. These three fragments are from the region 13q12-13q22, the chromosome region which contains the retinoblastoma ...
G. A. P. Bruns +3 more
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Definition of DRw10 by restriction fragment length polymorphism
Tissue Antigens, 1989To better define the presence of the DRw10 haplotype which has sometimes proved difficult to type by using serologic reagents, Southern blot analysis was performed on seven DRw10 heterozygous individuals with rheumatoid arthritis. Using the restriction enzymes Taq I or BamH I, the restriction fragment length polymorphism (RFLP) pattern for the DRw10 ...
Antonio Núñez-Roldán +4 more
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