Results 301 to 310 of about 562,575 (339)

Restriction Fragment Length Polymorphism Analysis

Current Protocols in Human Genetics, 1994
AbstractThis unit describes Restriction Fragment Length Polymorphism (RFLP) analysis, which utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA regions of interest. When investigating families for inheritance of an RFLP, aliquots of genomic DNA from individual family members are digested to completion with
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Optimization of Restriction Fragment DNA Mapping

Journal of Computational Biology, 1998
Consider a mapping project in which overlap of clonal segments is inferred from complete multiple restriction digests. The fragment sizes of the clones are measured with some error, potentially leading to a map with erroneous links. The number of errors in the map depends on the number and types of enzymes used to characterize the clones.
A F, Siegel, J C, Roach, C, Magness, E, Thayer, G, van den Engh   +4 more
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Multiplex-terminal restriction fragment length polymorphism

Nature Protocols, 2006
A novel method called "multiplex-terminal restriction fragment length polymorphism (M-TRFLP)" has been recently developed which can be used for simultaneous analysis of the community composition of two or more microbial taxa (up to four). This method can also be used for microbial diagnostic purposes. For M-TRFLP analysis, primers specific to different
Singh, Brajesh K. (R15253), Thomas, Nadine   +1 more
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Chromatographic separation of DNA restriction fragments

Journal of Chromatography B: Biomedical Sciences and Applications, 1987
Reversed-phase liquid chromatography (RPC-5) was the first chromatographic technique to be successfully applied to DNA restriction fragments. Size-exclusion high-performance liquid chromatography (HPLC) has the advantage of wide variability in buffer conditions but is restricted to an upper size limit at 800 base pairs.
R, Hecker, D, Riesner
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Renin locus restriction fragment length polymorphism

Human Genetics, 1989
The use of two genomic EcoRI fragments as probes is discussed.
L Z, Chen, S, Easteal
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Telomeric Terminal Restriction Fragment (TRF)

2003
Telomeres are protein-DNA structures at the ends of all eukaryotic chromosomes that are maintained by a unique ribonucleoprotein known as telomerase. This highly specialized RNA-dependent DNA polymerase provides a critical solution to the end-replication problem by adding TTAGGG repeats to 3' ends of human chromosomes (1-3). Telomere regulation is both
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Chromosome 13 restriction fragment length polymorphisms

Human Genetics, 1984
The gene locus for hereditary retinoblastoma is on human chromosome 13, band q14. With this gene localization in mind, we cloned DNA fragments from this chromosome. Three of the fragments identify restriction fragment length polymorphisms. These three fragments are from the region 13q12-13q22, the chromosome region which contains the retinoblastoma ...
T P, Dryja, J M, Rapaport, R, Weichselbaum, G A, Bruns   +3 more
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Immunoglobulin genes in DNA restriction fragments

Biochemistry, 1978
We have investigated the organization of immunoglobulin genes in mice. High molecular weight DNA from myelomas and Krebs ascites cells was cleaved with EcoRI restriction endonuclease and fractionated using preparative agarose gel electrophoresis. Each fraction was then hybridized to an immunoglobulin mRNA or a cDNA transcribed from the mRNA.
R, Wilson, A M, Walfield, A, Murakami, U, Storb   +3 more
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Restriction fragment length polymorphism

1988
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA.
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Restriction Fragment Length Polymorphism Typing of Mycobacteria

2003
In principle, restriction fragment length polymorphism (RFLP) typing can be applied to strains of all mycobacterial species for which suitable probes have been identified. International consensus has been achieved regarding the methodology of IS6110 RFLP typing of Mycobacterium tuberculosis complex isolates (1) and IS1245 RFLP typing of Mycobacterium ...
van Soolingen, Drivm, de Haas, PEW, Kremer, Krivm   +2 more
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