Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil [PDF]
Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum
Adolphe Zeze+34 more
core +1 more source
We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić+31 more
wiley +1 more source
Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin [PDF]
Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat ...
Chai, Lay Ching+8 more
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Revisiting Synthesis for One-Counter Automata
We study the (parameter) synthesis problem for one-counter automata with parameters. One-counter automata are obtained by extending classical finite-state automata with a counter whose value can range over non-negative integers and be tested for zero ...
Pérez, Guillermo A., Raha, Ritam
core
Prevalence of the vanB2 gene cluster in VanB glycopeptide-resistant enterococci in the United Kingdom and the Republic of Ireland and its association with a Tn5382-like element [PDF]
A total of 204 vanB enterococcal isolates, isolated between 1989 and 1999 from patients in 59 different hospitals in England, Wales, and Scotland and from a single hospital in the Republic of Ireland, were examined.
McGregor, Karen F.+3 more
core +1 more source
The authors applied joint/mixed models that predict mortality of trifluridine/tipiracil‐treated metastatic colorectal cancer patients based on circulating tumor DNA (ctDNA) trajectories. Patients at high risk of death could be spared aggressive therapy with the prospect of a higher quality of life in their remaining lifetime, whereas patients with a ...
Matthias Unseld+7 more
wiley +1 more source
A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers [PDF]
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required.
Brugmans, B.W.+4 more
core +2 more sources
This study simultaneously investigated circulating tumor cells (CTCs) and exosomes from small‐cell lung cancer (SCLC) patients. The elevated expression of JUNB and CXCR4 in CTCs was a poor prognostic factor for SCLC patients, whereas exosomal overexpression of these biomarkers revealed a high discrimination ability of patients from healthy individuals,
Dimitrios Papakonstantinou+13 more
wiley +1 more source
Peripheral blood proteome biomarkers distinguish immunosuppressive features of cancer progression
Immune status significantly influences cancer progression. This study used plasma proteomics to analyze benign 67NR and malignant 4T1 breast tumor models at early and late tumor stages. Immune‐related proteins–osteopontin (Spp1), lactotransferrin (Ltf), calreticulin (Calr) and peroxiredoxin 2 (Prdx2)–were associated with systemic myeloid‐derived ...
Yeon Ji Park+6 more
wiley +1 more source
Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis [PDF]
A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli.
Nebbia, Patrizia+3 more
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