Results 51 to 60 of about 288,437 (264)

IS6110 restriction fragment length polymorphism typing of Mycobacterium tuberculosis isolates from East Azerbaijan Province of Iran

open access: yesMemorias do Instituto Oswaldo Cruz, 2006
To investigate the genetic variation among Mycobacterium tuberculosis isolates in the East Azerbaijan Province of Iran and to evaluate the level of and risk factors for recent transmission of tuberculosis (TB), we performed IS6110-based restriction ...
Mohamad Asgharzadeh   +6 more
doaj   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

open access: yesMolecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués   +3 more
wiley   +1 more source

Study of Genetic Polymorphism in the Mitochondrial DNA of the Mediterranean Fruit Fly Ceratitis Capitata (Wiedemann, 1824) (Diptera:Tephritidae)Using the Method of PCR-RFLP in Three Governorates in the Middle Region of Iraq.

open access: yesIbn Al-Haitham Journal for Pure and Applied Sciences, 2017
Genetic polymorphism in a fragment of NADH (ND4),400bp long from the Mitochondrial DNA (mtDNA) of Mediterranean fruit fly ceratitis capitata (Wiedemann, 1824) using PCR-RFLP method with the restriction enzyme EcoRV in samples collected from three ...
M.M. Jawad, H.S. Al-Asady, I.A. Hussein
doaj  

MOLECULAR CHARACTERIZATION OF ‘Candidatus PHYTOPLASMA MALI’ STRAINS FROM BULGARIA AND POLAND

open access: yesActa Scientiarum Polonorum: Hortorum Cultus, 2019
During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of
Mirosława Cieślińska   +1 more
doaj   +1 more source

Methylation biomarkers can distinguish pleural mesothelioma from healthy pleura and other pleural pathologies

open access: yesMolecular Oncology, EarlyView.
We developed and validated a DNA methylation–based biomarker panel to distinguish pleural mesothelioma from other pleural conditions. Using the IMPRESS technology, we translated this panel into a clinically applicable assay. The resulting two classifier models demonstrated excellent performance, achieving high AUC values and strong diagnostic accuracy.
Janah Vandenhoeck   +12 more
wiley   +1 more source

Restriction fragment length polymorphism (RFLP) [PDF]

open access: yesAmerican Journal of Physical Anthropology, 1989
A restriction fragment length polymorphism (RFLP) is defined by an enzyme, a restriction endonuclease, that cuts the doublestranded DNA at a particular sequence of bases, a probe, a labeled, complementary segment of DNA that will anneal to a portion of the digested sample, and a set of variable fragment length bands that appear on a Southern blot.
openaire   +1 more source

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

open access: yesMolecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto   +8 more
wiley   +1 more source

Molecular Determination of Fasciola Spp. Isolates from Domestic Ruminants Fecal Samples in the Northwest of Iran

open access: yesIranian Journal of Parasitology, 2017
Background: Fasciola species are the main causes for fascioliasis with great financial losses and are among the most important food/water-borne parasites worldwide.
Abbas IMANI BARAN   +2 more
doaj  

An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species

open access: yesMolecules, 2017
The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
doaj   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

open access: yesMolecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice   +16 more
wiley   +1 more source

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