Results 61 to 70 of about 553,743 (341)
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Species Differentiation Of Fish Samples By Restriction Fragment Length Polymorphism Analysis Of Cytochrome B Gene [PDF]
, 2009 Metode pengukuran polimorfisme fragmen hasil pemotongan produkreaksi polimorfik berantai oleh enzim restriksi spesifik (polymerase chainreaction-restriction fragment length polymorphism, RFLP-PCR) telah digunakanuntuk membedakan beberapa jenis ikan ...
I, Z. M. (Zuraini) +3 more
core
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
Ribogospodarsʹka Nauka Ukraïni, 2013 The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency
Yu. Rud
doaj +1 more source
Do Hard SAT-Related Reasoning Tasks Become Easier in the Krom Fragment?
, 2018 Many reasoning problems are based on the problem of satisfiability (SAT). While SAT itself becomes easy when restricting the structure of the formulas in a certain way, the situation is more opaque for more involved decision problems.
Creignou, Nadia +2 more
core +3 more sources
The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells
FEBS Letters, EarlyView.The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman +3 more
wiley +1 more source
Биопрепараты: Профилактика, диагностика, лечениеINTRODUCTION. Chikungunya virus (CHIKV) genotyping involves sequencing fractions of genes encoding E1, E2, and nsP1 proteins or the entire genome of the virus.
N. A. Netesova +6 more
doaj +1 more source
A Method for Introducing Mutations into Large Vectors
Chinese Journal of Lung Cancer, 2014 Background and objective In vitro site-directed mutagenesis is a routine technique in molecular biology labs. However, although there are numbers of related methods available, most of these methods are not suitable for introducing mutations into large ...
Fanrong MENG +3 more
doaj +1 more source
MITOCHONDRIAL DNA POLYMORPHISMS AND FERTILITY IN BEEF CATTLE [PDF]
, 2002 Two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 422 beef cattle of Hereford and composite breeds from Wokalup’s ...
., Sutarno +3 more
core
DIGICALC: a restriction fragment analysis program
Nucleic Acids Research, 1986 DIGICALC is a program designed to aid in the acquisition, storage, and analysis of nucleic acid restriction fragment data. The chief considerations during program design were (i) ease of use for people with varying degrees of computer experience, (ii) minimal hardware requirements (e.g.
openaire +4 more sources