Results 131 to 140 of about 3,830 (170)
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Retinyl ester hydrolases in retinal pigment epithelium
Archives of Biochemistry and Biophysics, 1991In bovine retinal pigment epithelium membranes we have found three hydrolases which were active against trans-retinyl palmitate. This was possible by assaying different subcellular fractions as a function of pH in the range 3-9. Detection of these activities has been favored by the use in the enzyme assay of Triton X-100, which has an activating effect
M C, Gueli +6 more
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Retinyl esterase activity of purified rat liver retinyl ester lipoprotein complex
Archives of Biochemistry and Biophysics, 1979Abstract Retinyl ester lipoprotein complex from rat liver was shown to possess a retinyl esterase activity toward its own ligand complement. In the presence of serum albumin the retinyl esterase activity at 30 °C was about fivefold larger than the activity at 4 °C, while higher temperatures than 30 °C led to some degradation of retinyl compounds. The
C C, Chen, J, Heller
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The effect of dietary retinyl palmitate on the retinyl ester content of human or rat liver tumours
Food Additives and Contaminants, 1990(1990). The effect of dietary retinyl palmitate on the retinyl ester content of human or rat liver tumours. Food Additives & Contaminants: Vol. 7, No. sup1, pp. S69-S72.
A, Periquet +5 more
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Hydrolysis of cis and trans isomers of retinyl palmitate by retinyl ester hydrolase of pig liver
Archives of Biochemistry and Biophysics, 1988Relative retinyl ester hydrolase activities of pig liver homogenates (n = 4) toward 9,13-cis-, 13-cis-, 9-cis-, and all-trans-retinyl palmitate were 6.8 +/- 0.5 (SE), 5.7 +/- 0.5, 2.4 +/- 0.1, and 1, respectively. The range of apparent Km values for the four isomers was 142 to 268 microM, and the pH optima were 8-9 in all cases.
D A, Cooper, J A, Olson
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Properties of liver retinyl ester hydrolase in young pigs
Biochimica et Biophysica Acta (BBA) - General Subjects, 1986A new sensitive method for the assay of retinyl ester hydrolase in vitro was developed and applied to liver homogenates of 18 young pigs with depleted-to-adequate liver vitamin A reserves. Radioactive substrate was not required, because the formation of retinol could be adequately quantitated by reversed-phase high-performance liquid chromatography ...
D A, Cooper, J A, Olson
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HEPATIC STORES OF RETINOL AND RETINYL ESTERS IN ELDERLY PEOPLE
Age and Ageing, 1988Post-mortem concentrations of hepatic retinol and retinyl esters were determined in 40 subjects aged over 65 years to assess the effects of disease and malnutrition on vitamin A reserves. Three groups of patients (mean age 79.6 years) were studied: (1) previously healthy, (2) chronically ill, (3) chronically ill and wasted.
D A, Black, E, Heduan, D, Mitchell
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Retinyl ester hydrolase and the visual cycle in the chicken eye
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1995The ability of chicken retina and retinal pigment epithelium (RPE) membrane to hydrolyze vitamin A esters ([9,10(-3)H]all-trans- and 11-cis-retinyl palmitate) was studied. Hydrolytic activity within the retina was optimal at acidic pH of 5.0, whereas in the RPE significant hydrolytic activity was exhibited over a broad range of hydrogen ion ...
J J, Bustamante +3 more
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Retinol and retinyl esters in patients with alcoholic liver disease
Journal of Hepatology, 1989Liver retinoid levels and the retinyl esters were examined in liver biopsy specimens from 70 patients with alcoholic and nonalcoholic liver diseases. There was a wide variation in the liver retinoid levels. The liver retinoid level was statistically significantly lower in 15 patients with alcoholic liver disease and a depressed Normotest (NT) value of ...
H, Bell +5 more
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Effect of stanol ester on postabsorptive squalene and retinyl palmitate
Metabolism, 2000Stanol ester dissolved in margarine inhibits cholesterol absorption in general and, despite increasing cholesterol synthesis, decreases serum total and low-density lipoprotein (LDL) cholesterol levels, but its effects on postprandial lipid metabolism are unknown.
H, Relas, H, Gylling, T A, Miettinen
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The Reversed-Phase HPLC Behavior of Retinyl Esters
Journal of Liquid Chromatography, 1989Abstract Fifteen retinyl esters were synthesized, including a number of esters containing naturally occurring polyunsaturated fatty acids. The esters were purified by HPLC and their chromatographic behavior on reversed phase HPLC was studied. A plot of Log k' versus % water in the eluting solvent for each ester was very close to linear in the range ...
Hubert E. May, Sung I. Koo
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