Results 41 to 50 of about 123,458 (267)

Establishment of a resource recycling strategy by optimizing isobutanol production in engineered cyanobacteria using high salinity stress

Biotechnology for Biofuels, 2021
Background Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofriendly refining process.
Xiao-Xi Wu, Jian-Wei Li, Su-Fang Xing, Hui-Ting Chen, Chao Song, Shu-Guang Wang, Zhen Yan   +6 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Magnetic microsphere-based portable solid phase extraction device for on-site pre-concentration of organics from large-volume water samples

Scientific Reports, 2017
In this research a new magnetic material called M88 was fully synthetized and characterized for the extraction of pharmaceutical and personal care products in water samples.
Zhijian Yao, Qingqing Zhao, Yan Ma, Wei Wang, Qing Zhou, Aimin Li   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Data-driven approaches linking wastewater and source estimation hazardous waste for environmental management

Nature Communications
Industrial enterprises are major sources of contaminants, making their regulation vital for sustainable development. Tracking contaminant generation at the firm-level is challenging due to enterprise heterogeneity and the lack of a universal estimation ...
Wenjun Xie, Qingyuan Yu, Wen Fang, Xiaoge Zhang, Jinghua Geng, Jiayi Tang, Wenfei Jing, Miaomiao Liu, Zongwei Ma, Jianxun Yang, Jun Bi   +10 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A nanoscale observation to explain the discrepancy of electron exchange capacities between biochar containing comparable surface redox-active moieties

Biochar, 2022
Highlights The microscopic distribution of RAMs on biochar was achieved by combining C-AFM and a mediated electrochemical method. The uneven distribution of RAMs enabled biochar to possess outstanding EEC by enhancing accessibility.
Shasha Li, Liming Shao, Hua Zhang, Xuemin Lu, Fan Lü, Pinjing He   +5 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Electron transfer mediated activation of periodate by contaminants to generate 1O2 by charge-confined single-atom catalyst

Nature Communications
The electron transfer process (ETP) is able to avoid the redox cycling of catalysts by capturing electrons from contaminants directly. However, the ETP usually leads to the formation of oligomers and the reduction of oxidants to anions.
Qianqian Tang, Bangxiang Wu, Xiaowen Huang, Wei Ren, Lingling Liu, Lei Tian, Ying Chen, Long-Shuai Zhang, Qing Sun, Zhibing Kang, Tianyi Ma, Jian-Ping Zou   +11 more
doaj   +1 more source