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Retroelements, reverse transcriptase and evolution
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1995Retroelements are genetic elements that can exist as DNA or RNA or DNA/RNA duplexes. Although retroviruses are the best known retroelements, there are many other types, including close relatives of retroviruses like LTR retrotransposons, more distant relatives like non-LTR retrotransposons, caulimoviruses and hepadnaviruses and elements with virtually ...
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Reverse Transcriptase: Mediator of Genomic Plasticity
Virus Genes, 1995Reverse transcription has been an important mediator of genomic change. This influence dates back more than three billion years, when the RNA genome was converted into the DNA genome. While the current cellular role(s) of reverse transcriptase are not yet completely understood, it has become clear over the last few years that this enzyme is still ...
Jürgen Brosius, Henri Tiedge
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HIV-1 reverse transcriptase inhibitors
Applied Microbiology and Biotechnology, 2007Reverse transcriptase (RT) is one of the three enzymes encoded by the human immunodeficiency virus type 1 (HIV-1), the etiological agent of AIDS. Together with protease inhibitors, drugs inhibiting the RNA- and DNA-dependant DNA polymerase activity of RT are the major components of highly active antiretroviral therapy (HAART), which has dramatically ...
El Safadi, Yazan +2 more
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Telomerase Activity of Reverse Transcriptase
Science, 1997In their report “Reverse transcriptase motifs in the catalytic subunit of telomerase” ([25 Apr., p. 561][1]), Joachim Lingner et al . demonstrate that such motifs are present in the catalytic subunit of the telomerase that they purified and identified in Euplotes aediculatus .
Ricchetti, Miria, Buc, Henri
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Analytical Biochemistry, 1993
A chemiluminescent assay for reverse transcriptase (RT) of the human immunodeficiency virus 1 was developed using biotin-labeled oligodeoxythymidylic acid (biotin oligo-dT) and digoxigenin-deoxyuridine triphosphate instead of tritiated thymidine triphosphate. After the RT reaction, the newly polymerized strand from biotin oligo-dT contained digoxigenin
Kazuo Suzuki +3 more
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A chemiluminescent assay for reverse transcriptase (RT) of the human immunodeficiency virus 1 was developed using biotin-labeled oligodeoxythymidylic acid (biotin oligo-dT) and digoxigenin-deoxyuridine triphosphate instead of tritiated thymidine triphosphate. After the RT reaction, the newly polymerized strand from biotin oligo-dT contained digoxigenin
Kazuo Suzuki +3 more
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1981
Publisher Summary This chapter deals with the purification, enzymatic activities, mechanism, and research applications of reverse transcriptase to molecular biology. Reverse transcriptase transcribes RNA into complementary DNA (cDNA). It exhibits three enzymatic activities—namely, (1) conversion of RNA into DNA (RNA-dependent DNA polymerase), (2 ...
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Publisher Summary This chapter deals with the purification, enzymatic activities, mechanism, and research applications of reverse transcriptase to molecular biology. Reverse transcriptase transcribes RNA into complementary DNA (cDNA). It exhibits three enzymatic activities—namely, (1) conversion of RNA into DNA (RNA-dependent DNA polymerase), (2 ...
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A reverse transcriptase activity in potato mitochondria
Plant Molecular Biology, 1996A reverse transcriptase activity has been detected in potato mitochondria using special RNAs as templates: a bacterial RNA coding for neomycin phosphotransferase (neo pa RNA) and a Neurospora crassa mitochondrial RNA (184 nt RNA). Surprisingly, no exogenous primer addition was required.
Alejandra Moenne +2 more
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[27] Assays for retroviral reverse transcriptase
1995Publisher Summary This chapter summarizes some of the popular assays for reverse transcriptase (RT) and their use in quantifying levels of viral particles, viral RNA templates, and mutant RTs with altered polymerase and nuclease activities. The enzyme responsible for retroviral DNA synthesis, reverse transcriptase (RT) is both, a DNA polymerase and a
Stephen P. Goff +2 more
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