Results 91 to 100 of about 275,235 (344)

Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR

BMC Biotechnology, 2006
Background In order to overcome genomic DNA contamination in transcriptional studies, reverse template-specific polymerase chain reaction, a modification of reverse transcriptase polymerase chain reaction, is used.
Lindblad Peter, Svensson Håkan, Pinto Fernando   +2 more
doaj   +1 more source

Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis [PDF]

Korean Journal of Pediatrics, 2015
PurposeMeningitis is among the most common infections affecting the central nervous system. It can be difficult to determine the exact pathogen responsible for the infection and patients are often treated with empiric antibiotics.
Dahee Jin, Tae Hoon Heo, Jung Hye Byeon, Gun-Ha Kim, Mi Kyung Kim, So-Hee Eun, Baik-Lin Eun   +6 more
doaj   +1 more source

Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach [PDF]

, 2019
Sequencing very long stretches of the HIV-1 genome can advance studies on virus evolution and in vivo recombination but remains technically challenging.
Demecheleer, Els, Hardy, Jarryt, Hebberecht, Laura, Mortier, Virginie, Schauvliege, Marlies, Staelens, Delfien, Vancoillie, Leen, Verhofstede, Chris   +7 more
core   +1 more source

Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

Advanced Science, EarlyView.
The development of a novel engineered strain of Lactiplantibacillus plantarum (Lp) is reported that can produce bacterial extracellular vesicles (BEVs) at >60‐fold higher yields than the unmodified parental strain. These Lp BEVs retain therapeutic bioactivity as validated in a mouse model of dextran sodium sulfate‐induced colitis.
Nicholas H Pirolli, Daniel Levy, Alyssa Schledwitz, Natalia Sampaio Moura, Mitali Sarkar, Talia J. Solomon, Emily H. Powsner, Raith Nowak, Zuzanna Mamczarz, Christopher J. Bridgeman, Sulayman Khan, Andrew Hui, Nidhi Anne, Laura Reus, William E. Bentley, Jean‐Pierre Raufman, Steven M. Jay   +16 more
wiley   +1 more source

West Nile Virus Encephalitis in a Barbary Macaque (Macaca sylvanus)

Emerging Infectious Diseases, 2004
An aged Barbary ape (Macaca sylvanus) at the Toronto Zoo became infected with naturally acquired West Nile virus (WNV) encephalitis that caused neurologic signs, which, associated with other medical problems, led to euthanasia. The diagnosis was based on
Rolf-Arne Ølberg, Ian K. Barker, Graham J. Crawshaw, Mads F. Bertelsen, Michael A. Drebot, Maya Andonova   +5 more
doaj   +1 more source

Evidence for regulated expression of Telomeric Repeat-containing RNAs (TERRA) in parasitic trypanosomatids [PDF]

, 2017
The Telomeric Repeat-containing RNAs (TERRA) participate in the homeostasis of telomeres in higher eukaryotes. Here, we investigated the expression of TERRA in Leishmania spp.
Damasceno, Jeziel D., Silva, Gabriel L.A., Tosi, Luiz R.O., Tschudi, Christian   +3 more
core   +1 more source

WDR5‐H3K4me3 Epigenetic Axis Promotes TRMT6‐Dependent tRNA M1A Modification to Facilitate Triple‐Negative Breast Cancer Progression by Suppressing Ferroptosis

Advanced Science, EarlyView.
Upregulated TRMT6 forms aberrant hypermethylation of a specific tRNA pool and serves as a predictor of poor prognosis in TNBC. This m1A modification in tRNAs enhances translation of FTH1 and FTL, reducing the pool of bioavailable Fe2⁺. Reduced Fe2+ availability impairs RSL3‐induced lipid peroxidation and tumor progression.
Yuqing Lei, Xue Wang, Jiahe Liu, Zixu Niu, Jiaqi Hua, Qian Guo, Dan Du, Yuanchang Zhu, Fucheng He, Mingxia Zhou, Hongle Li, Jing He, Jun Li   +12 more
wiley   +1 more source

Studies of FLT3 mutations in paired presentation and relapse samples from patients with acute myeloid leukemia: implications for the role of FLT3 mutations in leukemogenesis, minimal residual disease detection, and possible therapy with FLT3 inhibitors [PDF]

, 2002
FLT3 mutations, either internal tandem duplications (ITDs) or aspartate residue 835 (D835) point mutations, are present in approximately one third of patients with acute myeloid leukemia (AML) and have been associated with an increased relapse rate.
Bowen, D.T., Frew, M.E., Gale, R.E., Kottaridis, P.D., Langabeer, S.E., Linch, D.C.   +5 more
core   +1 more source

Antiemesis Corticosteroids Potentiate Checkpoint Blockade Efficacy by Normalizing the Immune Microenvironment in Metastatic Murine Breast Cancer

Advanced Science, EarlyView.
Corticosteroids improve the efficacy of immune checkpoint blockade therapy in metastatic murine breast cancer. By normalizing the tumor immune microenvironment, corticosteroids reduce immunosuppressive signals, restore T‐cell function, and promote antitumor immune responses, resulting in enhanced tumor control.
John D. Martin, Koji Nagaoka, Myrofora Panagi, Akihiro Hosoi, Fotios Mpkeris, Pengwen Chen, Thahomina T. Khan, Margaret R. Martin, Changbo Sun, Chrysovalantis Voutouri, Maria Louca, Panagiotis Papageorgis, Akira Sumiyoshi, Nobuhiro Nitta, Kazuyoshi Takeda, Ichio Aoki, Kazunori Kataoka, Triantafyllos Stylianopoulos, Kazuhiro Kakimi, Horacio Cabral   +19 more
wiley   +1 more source

Possible Central Nervous System Infection by SARS Coronavirus

Emerging Infectious Diseases, 2004
On day 22 of illness, generalized tonic-clonic convulsion developed in a 32-year-old woman with severe acute respiratory syndrome (SARS). Cerebrospinal fluid tested positive for SARS coronavirus (SARS-CoV) by reverse transcriptase–polymerase chain ...
Kwok-Kwong Lau, Wai-Cho Yu, Chung-Ming Chu, Suet-Ting Lau, Bun Sheng, K.Y. Yuen   +5 more
doaj   +1 more source