Results 81 to 90 of about 172,121 (249)

Matching TGF‐β1 Activation With Stress‐Responsive Charge‐Reversal Hydrogel Microspheres for the Treatment of Osteoarthritis

Exploration, EarlyView.
Based on the interaction between the weakly cross‐linked disulfide bonds that break under stress and charge‐reversal messenger molecules, we herein achieved nanoparticle charge reversal under stress and constructed a stress‐responsive charge‐reversal hydrogel microsphere system using microfluidic technology.
Feng Lin, Yiwen Xu, Lei Xiang, Yueliang Zhu, Yihan Li, Yaping Zhuang, Zengjie Zhang, Jiayu Chen, Heemin Kang, Ning Zhang, Xiaohua Yu, Wenguo Cui   +11 more
wiley   +1 more source

Immunological assays for chemokine detection in in-vitro culture of CNS cells

Biological Procedures Online, 2003
Herein we review the various methods currently in use for determining the expression of chemokines by CNS cells in vitro. Chemokine detection assays are used in conjuction with one another to provide a comprehensive, biologically relevant assessment of ...
Mahajan Supriya D., Schwartz Stanley A., Nair Madhavan P.N.   +2 more
doaj   +1 more source

Competitive Reverse-Transcriptase Polymerase Chain Reaction without an Artificial Internal Standard

Analytical Biochemistry, 1995
Advances in our understanding of molecular and cellular physiology necessitate that mRNA levels for specific growth factors and other rare transcripts be measured quantitatively in small samples. Conventional methods such as Northern blot analysis and solution hybridization/ribonuclease protection are not sufficiently sensitive.
M E, Zenilman, W, Graham, K, Tanner, A R, Shuldiner   +3 more
openaire   +2 more sources

Hyperoside alleviates endometrial stromal cell senescence in unexplained recurrent spontaneous abortion via DHX9‐mediated R‐loop resolution

iMeta, EarlyView.
This study suggests that the food‐derived flavonoid hyperoside may act as a natural DExH‐box helicase 9 (DHX9) modulator, directly targeting Thr419 of DHX9 to help resolve pathological R‐loop accumulation. Consequently, it suppresses cyclic GMP‐AMP synthase‐stimulator of interferon genes (cGAS‐STING)‐driven endometrial stromal senescence and restores ...
Yuepeng Jiang, Hongli Zhao, Xinyi Ding, Haoling Zhang, Yadong Guo, Yiming Ma, Lingyi Cai, Qingnan Fan, Ruisi Peng, Fuyuan Yang, Doblin Sandai, Xianling Cao, Jiali Yao, Wenyi Wang, Zhiheng Lin, Wangzheqi Zhang, Jialin He, Aihua Zhang, Xiaoxuan Zhao   +18 more
wiley   +1 more source

Laboratory Diagnosis of Mumps in a Partially Immunized Population: The Nova Scotia Experience

Canadian Journal of Infectious Diseases and Medical Microbiology, 2009
BACKGROUND: In 2007, Atlantic Canada experienced a large outbreak of mumps predominately in university students who had received a single dose of measles, mumps and rubella vaccine.
TF Hatchette, R Davidson, S Clay, J Pettipas, J LeBlanc, S Sarwal, M Smieja, KR Forward   +7 more
doaj   +1 more source

Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil

Memorias do Instituto Oswaldo Cruz, 2006
The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection.
Ana Maria Tavares Borges, José Marcus Sócrates Teixeira, Paulo Sérgio Sucasas da Costa, Loreny Gimenes Giugliano, Fabíola Souza Fiaccadori, Rachel de Carvalho e Franco, Wilia Marta Elsner Diederichsen de Brito, José Paulo Gagliardi Leite, Divina das Dores de Paula Cardoso   +8 more
doaj   +1 more source

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Cerebrospinal fluid fistula as a complication of reverse transcriptase-polymerase chain reaction collection for the detection of coronavirus disease 2019: illustrative cases. [PDF]

J Neurosurg Case Lessons, 2023
Palavani LB, Andrade CVF, Andrade RA, Alves E, Barros MF, Barbieri JF.   +5 more
europepmc   +1 more source

Myeloid sarcoma presenting as an isolated pancreatic mass in a 3‐year‐old child

JPGN Reports, EarlyView.
Abstract Myeloid sarcoma (MS) is an extramedullary tumor of myeloid precursor cells, frequently associated with acute myeloid leukemia (AML), and rarely occurring in isolation. We present a child with obstructive jaundice secondary to a pancreatic head mass.
Jappmann Kaur Monga, Cara L. Mack, Michelle Saad, Dylan Applin, Maisam Abu‐El‐Haija, David Vitale, Zachary Graff, Gila Ginzburg   +7 more
wiley   +1 more source