A new approach to understanding T cell development: the isolation and characterization of immature CD4-, CD8-, CD3- T cell cDNAs by subtraction cloning [PDF]
During T cell development in the mammalian thymus, immature T cells are observed that lack the cell surface markers CD4, CD8, and CD3. A subtracted cDNA library was constructed to isolate cDNAs that are specific for these immature T cells.
Gese, Elaine, Hood, Leroy, Orr, Sally L.
core
Pathogenic DMD variants usually follow the reading‐frame rule: out‐of‐frame changes cause Duchenne muscular dystrophy, whereas in‐frame ones produce Becker muscular dystrophy (BMD). We report a 23‐year‐old man with BMD‐like weakness, calf hypertrophy, elevated creatine kinase, and dilated cardiomyopathy.
Hiroya Naruse +16 more
wiley +1 more source
Case series - COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis. [PDF]
Masterson JM +9 more
europepmc +1 more source
Short communication: optimization of semi-quantitative RT PCR analysis for CPT I gene expression in rainbow trout (Oncorhynchus mykiss) [PDF]
A key enzyme in mitochondrial β¬oxidation, carnitine palmitoyltransferase (CPT) I, is transcriptionally regulated in mammals, but this enzyme also experiences allosteric modulations (Harano et al., 1985; Murthy and Pande, 1987; Bezaire et al., 2004). CPT
Jalali, S.A.H. +3 more
core
Encapsulated acriflavine (ACF) and copper oxide nanoparticles (CuO NPs) are coreleased from coaxial nanofibers, producing synergistic antiglioblastoma multiforme effects. ACF suppresses the hypoxia‐inducible factor 1α pathway related to many downstream factors for tumor growth, while CuO NPs generate reactive oxygen species that assist cuproptosis ...
Daewoo Han +4 more
wiley +1 more source
Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase-Polymerase Chain Reaction [PDF]
B.P. Shankar +5 more
openalex +1 more source
Mucin Glycoprotein Nanoparticles Enable a Selective Antisense Therapy for Oncogenic MicroRNAs
Mucin glycoproteins are turned into nanoparticles by employing synthetic DNA strands, which have a dual function: they stabilize the nanoparticles and act as binding sites for intracellular miRNA‐21. Thus, upon internalization into tumor cells, these mucin nanoparticles can deplete miRNA‐21 from the cytosol, which induces apoptosis in vitro and in vivo.
Ceren Kimna +9 more
wiley +1 more source
Parechovirus and enteroviruses among young infants with sepsis in Iran
Background and Objectives: Human parechoviruses (HPeV) and Human enteroviruses (EV) frequently cause a sepsis-like illness in young infants (younger than three months).
Manoochehr Makvandi +6 more
doaj
A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction [PDF]
Adriene Siqueira de Melo +3 more
openalex +1 more source

