Results 151 to 160 of about 171,737 (259)

Degradomics for large‐scale mechanistic insights on proteases and proteolysis in human health

The FEBS Journal, EarlyView.
Proteolysis has an important role in human disease but remains relatively unexplored. Degradomics, the uncovering of proteolysis in tissues, cells, and proteins, uses mass spectrometry‐based terminomics to identify protein termini occurring therein (forward degradomics) and to define the actions of proteases (reverse degradomics).
Daniel R. Martin, Sumit Bhutada, Suneel S. Apte   +2 more
wiley   +1 more source

Improvement of a RT-PCR assay for Yellow Fever virus genome detection

Revista de Ciências Farmacêuticas Básica e Aplicada, 2019
The aim of the present study was to describe an improved protocol of reverse transcription polymerase chain reaction (RT-PCR) for Yellow Fever virus genome detection.
Tatiana Carneiro da Rocha, Mario Antônio Navarro-Silva, Ana Caroline Dalla Bona, Marcio Roberto Teixeira Nunes, Walfrido Kühl Svoboda, Eliane Carneiro Gomes   +5 more
doaj  

SARS-CoV-2 detection by reverse transcriptase polymerase chain reaction testing: Analysis of false positive results and recommendations for quality control measures. [PDF]

Pathol Res Pract, 2021
Layfield LJ, Camp S, Bowers K, Miller DC.   +3 more
europepmc   +1 more source

Identification of RS virus by using reverse transcriptase polymerase chain reaction.

Uirusu, 1991
Okamoto, Yoshitaka, Ito, Eiko, Shirotori, Kozi, Kudo, Kazuo, Togawa, Kiyoshi, Saito, Ichiro, Moro, Itaru   +6 more
openaire   +3 more sources

Homologous recombination mutants cause differing lethality between h− and h+ Schizosaccharomyces pombe strains due to mat1 heterochromatin

The FEBS Journal, EarlyView.
Homologous recombination (HR) in Schizosaccharomyces pombe is essential when mating‐type switching–induced double‐strand breaks (DSBs) form at the mat1 locus. In h90 and h− strains, efficient mat1 DSB formation renders HR indispensable unless suppressors block these breaks.
Peter Kolesar, Siarhei Paliavoi, Barbora Stefanovie, Jan Josef Palecek   +3 more
wiley   +1 more source

Clinical Performance of the Loop-Mediated Isothermal Amplification Method Compared With Reverse Transcriptase Polymerase Chain Reaction for COVID-19 Diagnosis in Indonesia and the Philippines. [PDF]

Cureus
Mizushima R, Suriapranata IM, Geraldino N, Kurniasih TS, Andriani F, Cucunawangsih, Wijaya RS, Lugito NP, Hernandez-Diwa M, Ang MA, Ozoa GM, Uy TCF, Tabunar SM, Germar MJ, Urbiztondo-Benedicto MT, Saito S, Yamamoto K, Sakaguchi M, Hosaka N, Koyano S, Siburian MD, Umano-Urbiztondo MR, Kobayashi T, Miura T, Ohmagari N.   +24 more
europepmc   +1 more source

Caffeylpyruvate hydrolase from the bioluminescent fungus Neonothopanus gardneri is the key recycling enzyme in the fungal bioluminescence pathway

The FEBS Journal, EarlyView.
Caffeic acid is a central metabolite in the fungal bioluminescence pathway. We identified and characterized caffeylpyruvate hydrolase from Neonothopanus gardneri (ngarCPH) and demonstrate its ability to hydrolyze fungal oxyluciferin into caffeic and pyruvic acids, confirming a complete and self‐sustained fungal bioluminescence cycle.
Caio K. Zamuner, Douglas M. M. Soares, Bianca B. Nóbrega, Etelvino J. H. Bechara, Zinaida M. Kaskova, Alexander S. Mishin, Karen S. Sarkisyan, Ilia V. Yampolsky, Cassius V. Stevani   +8 more
wiley   +1 more source

Evaluation of immunohistochemical and gene expression of Janus kinase 1 and Janus kinase 3 in the skin of different clinical types of mycosis fungoides patients - Part II: reverse transcriptase-polymerase chain reaction. [PDF]

An Bras Dermatol
El-Amawy HS, Mohamed Ali BM, Salem ML, Elgarhy L.   +3 more
europepmc   +1 more source

Detection of SARS-CoV2 virus using the real-time reverse transcriptase polymerase chain reaction in semen and seminal plasma from men with active COVID-19 infection - A pilot study. [PDF]

Indian J Urol, 2021
Gupta P, Choudhary A, Gopal G, Kumar R, Kumar A, Tiwari P, Malhotra N.   +6 more
europepmc   +1 more source

Mechanical stiffness orchestrates distinct regulation of MRTFs and YAP/TAZ transcriptional cofactors in hepatocytes

The FEBS Journal, EarlyView.
MRTFs and YAP/TAZ proteins, or mechanosensitive transcriptional cofactors (MRTcoF), regulate common genes associated with cellular contractility and immune cell infiltration. Their differential regulation in response to stiffness is linked to changes in hepatocyte's aspect ratio. MRTFB does not undergo nuclear translocation under these conditions. This
Brenda Selene Torres‐Ortiz, Daniel Pérez‐Calixto, Jorge Carretero‐Ortega, Aida Gabriela Guzman‐López, Diana Cristina Pinto‐Dueñas, Mariana Hernández‐Juárez, Alejandra Jiménez‐Escobar, Adriana Rodríguez‐Hernández, Beatriz Díaz‐Bello, Nathalia Serna‐Márquez, Miguel Ángel Peña‐Rico, Laura Gómez‐Romero, Francisco Gabriel Vázquez‐Cuevas, Genaro Vázquez‐Victorio   +13 more
wiley   +1 more source