Single amino acid substitutions in the global regulator CodY can alter nutrient sensing and virulence regulation in Clostridioides difficile. We show that the CodY(Y146N) and CodY(V58A) variants display altered ligand binding and reduced promoter binding, leading to derepression of toxin regulatory pathways and increased virulence in vivo.
Md Kamrul Hasan +2 more
wiley +1 more source
Validation of Endogenous Control Genes by Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction for Acute Leukemia Gene Expression Studies. [PDF]
Pessoa FMCP +12 more
europepmc +1 more source
Diagnostic Performance of CT and Reverse Transcriptase Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis. [PDF]
Kim H, Hong H, Yoon SH.
europepmc +1 more source
Comparison of High-Resolution Computed Tomography and Real-Time Reverse Transcriptase Polymerase Chain Reaction in Diagnosis of COVID-19 Pneumonia in Intensive Care Unit Population. [PDF]
Murtaza HG +7 more
europepmc +1 more source
Cuscuta reflexa infection sites. Summary Cuscuta species are holoparasitic plants that penetrate host stems with haustoria, connect to the vasculature and exhaust water, nutrients and carbohydrates. Parasite seedlings possess only limited maternal reserves and the development of a vascular connection takes several days.
Maleen Hartenstein +10 more
wiley +1 more source
An Evaluation of the Sensitivity and Specificity of Three COVID-19 Rapid Immunochromatographic Test Kits Compared to Real-Time Reverse Transcriptase-Polymerase Chain Reaction (rRT-PCR) Among Clinical Samples. [PDF]
Bani S +9 more
europepmc +1 more source
Epidemiology, Outcomes, and Associated Factors of Coronavirus Disease 2019 (COVID-19) Reverse Transcriptase Polymerase Chain Reaction-Confirmed Cases in the San Pedro Sula Metropolitan Area, Honduras. [PDF]
Zuniga-Moya JC +15 more
europepmc +1 more source
Endogenous RALF peptide function is required for powdery mildew host colonization
Model of RALF‐FER‐dependent effects on powdery mildew sporulation. Summary The receptor kinase FERONIA (FER) is a susceptibility factor for biotrophic powdery mildew fungal pathogens in Arabidopsis thaliana, but the underlying molecular mechanisms remain largely unknown. FER is required for the perception of endogenous RAPID ALKALINIZATION FACTOR (RALF)
Henriette Leicher +17 more
wiley +1 more source
Direct detection of canine picornavirus complete coding sequence in wastewater using long-range reverse-transcriptase polymerase chain reaction and long-read sequencing. [PDF]
Faleye TOC +5 more
europepmc +1 more source
CNGCs in Marchantia paleacea uncouple arbuscular mycorrhizal symbiosis and rhizoid development
Rhizoid growth and AM fungal infection are uncoupled. Summary In Marchantia paleacea, MpaDMI1‐dependent nuclear Ca2+ oscillations are essential for arbuscular mycorrhizal (AM) fungal colonisation, indicating that endosymbiosis‐mediated nuclear Ca2+ signalling is a conserved feature of land plant–AM symbiosis.
Anson Ho Ching Lam +3 more
wiley +1 more source

