Standardization and application of a modified RFLP-PCR methodology for analysis of polymorphisms linked to treatment resistance in Ancylostoma braziliense. [PDF]
Single nucleotide polymorphisms (SNPs) in codons 167, 198 and 200 of the beta-tubulin isotype 1 gene are associated with benzimidazoles resistance in many helminths. Codon 167 mutation has never been described in hookworms; however, polymorphisms in codons 198 and 200 have been described for Ancylostoma caninum and Necator americanus.
Furtado LFV +2 more
europepmc +5 more sources
Molecular Typing of Methicillin Resistant Staphylococcus aureus using coa Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism: A Cross-sectional Study [PDF]
Introduction: Methicillin Resistant Staphylococcus aureus (MRSA) are the most important multidrug resistant pathogen of humans causing a wide array of infections.
P Hema, B Appalaraju, R Someshwaran
doaj +1 more source
PCR-RFLP analysis of rbcL chloroplast gene [PDF]
To identify intergeneric, interspecific, and intraspecific polymorphisms, PCR-RFLP studies of the chloroplast rbcL gene profiles were carried out using five restriction endonucleases (AluI, HpaII, BsuRI, BstHHI, BstMBI) in 14 species belonging to 6 ...
Sheikina Olga
doaj +1 more source
RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas [PDF]
The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3-phosphoshikimate-1-carboxyvinyltransferase, a key enzyme of aromatic amino acids and ...
A, Cascón Soriano +5 more
openaire +2 more sources
تمرکز ایزوالکتریک و روشهای متصل به PCR-RFLP برای کشف کمبود Alpha1-antitrypsin [PDF]
53 persons suspected to alpha1-antitrypsin deficiency detection (AATD) were investigated for ZZ, MZ, ZS, SS, and MS alleles analysis by serum protein electrophoresis (SPE), measurement of trypsin inhibiting capacity (TIC), isoelectric focusing (IEF ...
ب.غ. گودرزی +4 more
doaj +1 more source
Exploratory Visualization of RFLP-PCR Genomic Data Using Multidimensional Scaling [PDF]
Gel electrophoresis images are common results of biomolecular techniques such as RFLP-PCR (restriction fragment length polymorphism - polymerase chain reaction). These images are used to discover the genetic relations between organisms. Find patterns in these images are a complex and delayed work if it is performed by humans.
Soledad Espezúa Llerena +1 more
openaire +2 more sources
Maytenus aquifolia and Maytenus ilicifolia are native plants from South America and popularly known as ‘Espinheira-santa’. Both are used as tea due to their efficiency in the treatment of ulcer, gastritis and indigestion.
Sandra Sayuri Nakamura +4 more
doaj +1 more source
Background: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses.
Diego Rayan Teixeira de Sousa +8 more
doaj +1 more source
Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP
Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences ...
Ki-Rok Kwon, Seung-Il Baek, Suk Ho Choi
doaj +1 more source
Typing class I HLA-A gene using a nested PCR-RFLP procedure
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution ...
E.C. Castelli +3 more
doaj +1 more source

